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Production Process for Stem Cell Based Therapeutic Implants: Expansion of the Production Cell Line and Cultivation of Encapsulated Cells

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Bioreactor Systems for Tissue Engineering II

Part of the book series: Advances in Biochemical Engineering / Biotechnology ((ABE,volume 123))

Abstract

Cell based therapy promises the treatment of many diseases like diabetes mellitus, Parkinson disease or stroke. Microencapsulation of the cells protects them against host-vs-graft reactions and thus enables the usage of allogenic cell lines for the manufacturing of cell therapeutic implants. The production process of such implants consists mainly of the three steps expansion of the cells, encapsulation of the cells, and cultivation of the encapsulated cells in order to increase their vitality and thus quality. This chapter deals with the development of fixed-bed bioreactor-based cultivation procedures used in the first and third step of production. The bioreactor system for the expansion of the stem cell line (hMSC-TERT) is based on non-porous glass spheres, which support cell growth and harvesting with high yield and vitality. The cultivation process for the spherical cell based implants leads to an increase of vitality and additionally enables the application of a medium-based differentiation protocol.

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Acknowledgements

The authors would like to thank the Federal Ministry of Economics and Technology for financial support as well as the CellMed AG for providing the production cell line hMSC-TERT and the CellBeads®.

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Authors and Affiliations

  1. Institute of Biopharmaceutical Technology, University of Applied Sciences Giessen-Friedberg, Giessen, Germany

    C. Weber, S. Pohl, Pablo Pino-Grace, D. Freimark & P. Czermak

  2. Institute of Bioprocess and Biosystem Technology, University of Hamburg-Harburg, Hamburg, Germany

    R. Poertner

  3. CellMed AG, Alzenau, Germany

    C. Wallrapp & P. Geigle

  4. Department of Chemical Engineering, Kansas State University, Manhattan, KS, USA

    P. Czermak

Authors
  1. C. Weber
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  2. S. Pohl
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  3. R. Poertner
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  4. Pablo Pino-Grace
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  5. D. Freimark
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  6. C. Wallrapp
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  7. P. Geigle
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  8. P. Czermak
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Corresponding author

Correspondence to P. Czermak .

Editor information

Editors and Affiliations

  1. Inst. Technische Chemie, Universität Hannover, Callinstr. 3, Hannover, 30167, Germany

    Cornelia Kasper

  2. Klinische und Experimentelle, Traumatologie, Ludwig Boltzmann Institut für, Donaueschingenstr. 13, Wien, 1200, Austria

    Martijn van Griensven

  3. Inst.Biotechnologie u. Verfahrenstechnik, TU Hamburg-Harburg, Denickestr. 15, Hamburg, 21073, Germany

    Ralf Pörtner

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Weber, C. et al. (2010). Production Process for Stem Cell Based Therapeutic Implants: Expansion of the Production Cell Line and Cultivation of Encapsulated Cells. In: Kasper, C., van Griensven, M., Pörtner, R. (eds) Bioreactor Systems for Tissue Engineering II. Advances in Biochemical Engineering / Biotechnology, vol 123. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2009_25

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