About this series
The book series on BIOANALYSIS: Advanced Materials, Methods, and Devices is intended to serve as an authoritative reference source for a broad, interdisciplinary audience involved in the research, teaching, learning, and practice of bioanalytical science and technology. Bioanalysis has experienced explosive growth due to the dramatic convergence of advanced technologies and molecular biology research, which has led to the development of entirely new ways to probe biomolecular and cellular processes as well as biological responses to implanted biomaterials and engineered tissues. Novel optical techniques using a wide variety of reporter gene assays, ion channel probes, and fluorescent probes have provided powerful bioanalytical tools for cell-based assays. Fluorescent reporters allow the development of live cell assays with the ability for in vivo sensing of individual biological responses across cell populations, tracking the transport of biological species within intracellular environments, and monitoring multiple responses from the same cell. Novel classes of labels using inorganic fluorophors based on quantum dots or surface-enhanced Raman scattering labels provide unique possibilities for multiplex bioanalyses.
Laser-based technologies are important in the development of ultrasensitive bioanalytical techniques. Lasers are now used as excitation light sources in a wide variety of molecular bioassays. Today, single-molecule detection techniques using laser excitation provide the ultimate tools to elucidate cellular processes. The possibility of fabricating nanoscale materials and components has recently led to the development of devices and techniques that can measure fundamental parameters at the molecular level. With "optical tweezer" techniques, for example, small particles may be trapped by radiation pressure in the focal volume of a high-intensity, focused laser beam. Ingenious optical trapping systems have also been used to measure the force exerted by individual motor proteins.
Whereas the laser has provided a new technology for excitation, the miniaturization and mass production of sensor devices and their associated electronic circuitry has radically transformed the ways detection and imaging of biological species can be performed in vivo and ex vivo. Sensor miniaturization has enabled significant advances in imaging technologies over the last decade in such areas as microarrays and biochips for bioanalysis of a wide variety of species. The miniaturization of high-density optical sensor arrays has also led to the development of advanced high-resolution imaging methods at the cellular or molecular scales. With powerful microscopic tools using near-field optics, scientists are now able to image the biochemical processes and sub-microscopic structures of living cells at unprecedented resolutions.
Recently, nanotechnology, which involves research on and development of materials and species at length scales between 1 to100 nanometers, has been revolutionizing important areas in bioanalysis at the molecular and cellular level. The combination of molecular nanotechnology and various sensing modalities (optical, electrochemical, etc) opens the possibility of detecting and manipulating atoms and molecules using nano-devices, which have the potential for a wide variety of bioanalyses at the cellular level. These new bioanalytical tools are capable of probing the nanometer world and will make it possible to characterize the chemical and mechanical properties of biomolecules and cells, discover novel phenomena and processes, and provide science with a wide range of tools, materials, devices, and systems with unique characteristics.
This book series will present the most recent scientific and technological advances in materials, methods and instrumentation of interest to researchers, students, and manufacturers. The goal is to provide a comprehensive forum to integrate the contributions of biophysicists, biomedical engineers, materials scientists, chemists, chemical engineers, biologists, and others involved in the science and technology revolution reshaping molecular biology and biomedicine.