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  • © 2023

SILAC

Methods and Protocols

  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2603)

Buying options

Hardcover Book USD 219.99
Price excludes VAT (USA)
This title has not yet been released. You will be able to pre-order it soon.

About this book

This detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, SILAC: Methods and Protocols serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science.

Keywords

  • Quantitative proteomics
  • Stable isotope labeling
  • Bioinformatics
  • Functional proteomics
  • Mass spectrometry

Editors and Affiliations

  • Department of Analytical Chemistry, Complutense University of Madrid, Madrid, Spain

    Jose L. Luque-Garcia

Bibliographic Information

  • Book Title: SILAC

  • Book Subtitle: Methods and Protocols

  • Editors: Jose L. Luque-Garcia

  • Series Title: Methods in Molecular Biology

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: VIII, 292

  • Number of Illustrations: 10 b/w illustrations, 31 illustrations in colour

  • Topics: Proteins, Cytological Techniques

Buying options

Hardcover Book USD 219.99
Price excludes VAT (USA)
This title has not yet been released. You will be able to pre-order it soon.