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  • © 2023

Signal Transduction Immunohistochemistry

Methods and Protocols

  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2593)

Buying options

Hardcover Book USD 249.99
Price excludes VAT (USA)
This title has not yet been released. You will be able to pre-order it soon.

About this book

This new edition collects diverse protocols compiled by experts from different areas of research as well as by biotech researchers developing novel technologies in the area of immunohistochemistry (IHC). After a few chapters to help orient novices entering the biomedical arena, the book includes methods chapters covering multiplex IHC including fluorescence and chromogenic techniques, combination of IHC with In Situ Hybridization (ISH), wavelet transform approach for organelle tracking, transcription factors in human stem cells, differentiation of mesenchymal stem cells, 3D imaging, phenotyping of glial cells in the human brain, tissue fixation, permeabilization, and cryo-preservation, as well as other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Signal Transduction Immunohistochemistry: Methods and Protocols, Third Edition aims to arm novices and experts with vital protocols they can use either as-is or tailor them for specific experimental needs.


  • Antibodies
  • Multiplex IHC
  • Flow cytometry
  • Signal transduction targets
  • Drug discovery
  • Biotechnology

Editors and Affiliations

  • Bio-Techne, Minneapolis, USA

    Alexander E. Kalyuzhny

Bibliographic Information

  • Book Title: Signal Transduction Immunohistochemistry

  • Book Subtitle: Methods and Protocols

  • Editors: Alexander E. Kalyuzhny

  • Series Title: Methods in Molecular Biology

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: VIII, 371

  • Number of Illustrations: 17 b/w illustrations, 67 illustrations in colour

  • Topics: Immunological Techniques, Tissues

Buying options

Hardcover Book USD 249.99
Price excludes VAT (USA)
This title has not yet been released. You will be able to pre-order it soon.