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Bacterial and Archaeal Motility

  • Book
  • © 2023

Overview

Editors:
  1. Tohru Minamino

    1. Graduate School of Frontier Biosciences, Osaka University, Suita, Japan

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    You can also search for this editor in PubMed   Google Scholar

  2. Makoto Miyata

    1. Graduate School of Science, Osaka Metropolitan University, Osaka, Japan

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    You can also search for this editor in PubMed   Google Scholar

  3. Keiichi Namba

    1. Graduate School of Frontier Biosciences, Osaka University, Suita, Japan

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2646)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Bacterial Flagellar Protein Export and Assembly

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Purification of the Transmembrane Polypeptide Channel Complex of the Salmonella Flagellar Type III Secretion System

      • Miki Kinoshita, Keiichi Namba, Tohru Minamino
      Pages 3-15

    3. In Vitro Flagellar Type III Protein Transport Assay Using Inverted Membrane Vesicles

      • Katsumi Imada, Hiroyuki Terashima
      Pages 17-26

    4. Molecular Simulation to Investigate Open–Close Motion of a Flagellar Export Apparatus Protein FlhAC

      • Akio Kitao
      Pages 27-34

    5. Live-Cell Imaging of the Assembly and Ejection Processes of the Bacterial Flagella by Fluorescence Microscopy

      • Xiang-Yu Zhuang, Chao-Kai Tseng, Chien-Jung Lo
      Pages 35-42

    6. Purification and CryoEM Image Analysis of the Bacterial Flagellar Filament

      • Tomoko Yamaguchi, Tomoko Miyata, Fumiaki Makino, Keiichi Namba
      Pages 43-53

  3. Flagella-Driven Motility of Bacteria

    1. Front Matter

      Pages 55-55
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    2. Site-Specific Isotope Labeling of FliG for Studying Structural Dynamics Using Nuclear Magnetic Resonance Spectroscopy

      • Tatsuro Nishikino, Yohei Miyanoiri
      Pages 57-70

    3. Site-Directed Cross-Linking Between Bacterial Flagellar Motor Proteins In Vivo

      • Hiroyuki Terashima, Michio Homma, Seiji Kojima
      Pages 71-82

    4. Measurements of the Ion Channel Activity of the Transmembrane Stator Complex in the Bacterial Flagellar Motor

      • Yusuke V. Morimoto, Tohru Minamino
      Pages 83-94

    5. Purification of the Na+-Driven PomAB Stator Complex and Its Analysis Using ATR-FTIR Spectroscopy

      • Seiji Kojima, Michio Homma, Hideki Kandori
      Pages 95-107

    6. Purification of Na+-Driven MotPS Stator Complexes and Single-Molecule Imaging by High-Speed Atomic Force Microscopy

      • Naoya Terahara, Noriyuki Kodera
      Pages 109-124

    7. High-Resolution Rotation Assay of the Bacterial Flagellar Motor Near Zero Loads Using a Mutant Having a Rod-Like Straight Hook

      • Shuichi Nakamura, Tohru Minamino
      Pages 125-131

    8. Live-Cell Fluorescence Imaging of Magnetosome Organelle for Magnetotaxis Motility

      • Yukako Eguchi, Azuma Taoka
      Pages 133-146

    9. Swarming Motility Assays in Salmonella

      • Jonathan D. Partridge, Rasika M. Harshey
      Pages 147-158

    10. Analysis of Adhesion and Surface Motility of a Spirochete Bacterium

      • Shuichi Nakamura, Jun Xu, Nobuo Koizumi
      Pages 159-168

    11. Force Measurement of Bacterial Swimming Using Optical Tweezers

      • Keigo Abe, Kyosuke Takabe, Shuichi Nakamura
      Pages 169-179

  4. Archaella-Driven Motility of Archaea

    1. Front Matter

      Pages 181-181
      Download chapter PDF

    2. Archaella Isolation

      • Shamphavi Sivabalasarma, João N. de Sousa Machado, Sonja-Verena Albers, Ken F. Jarrell
      Pages 183-195
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Keywords

About this book

This detailed volume presents cutting-edge research protocols to study the structure and dynamics of bacterial and archaeal motility systems using bacterial genetics, molecular biology, biochemistry, biophysics, structural biology, cell biology, microscopy imaging, and molecular dynamics simulation. Beginning with a section on bacterial flagellar protein export and assembly, the book continues with chapters covering flagella-driven motility of bacteria, archaella-driven motility of archaea, type IV-driven twitching motility of bacteria, as well as adhesion-based gliding motility of bacteria and other unique motility systems. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and thorough, Bacterialand Archaeal Motility is the ideal reference for researchers working in this vital area of microbiology.

Editors and Affiliations

  • Graduate School of Frontier Biosciences, Osaka University, Suita, Japan

    Tohru Minamino, Keiichi Namba

  • Graduate School of Science, Osaka Metropolitan University, Osaka, Japan

    Makoto Miyata

Bibliographic Information

  • Book Title: Bacterial and Archaeal Motility

  • Editors: Tohru Minamino, Makoto Miyata, Keiichi Namba

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3060-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3059-4Published: 27 February 2023

  • Softcover ISBN: 978-1-0716-3062-4Published: 27 February 2023

  • eBook ISBN: 978-1-0716-3060-0Published: 26 February 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 402

  • Number of Illustrations: 24 b/w illustrations, 81 illustrations in colour

  • Topics: Microbiology, Microbiology

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