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The Tumor Microenvironment

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Josie Ursini-Siegel

    1. Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Canada

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2614)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Imaging Mass Cytometry in Immuno-Oncology

    • Tiziana Cotechini, Oliver Jones, Charles Colin Thomas Hindmarch
    Pages 1-15

  3. Analyzing the Tumor-Immune Microenvironment by Flow Cytometry

    • François Santinon, Yoon Kow Young, Sonia V. del Rincón, Koren K. Mann
    Pages 17-36

  4. Blood Sample Processing and Banking for Functional and Molecular Analyses

    • Paméla Thébault, Jean-François Cailhier, Réjean Lapointe
    Pages 37-46

  5. Quantitatively Assessing the Respiratory Burst in Innate Immune Cells

    • Ava L. Sargent, Jordan A. Leedberg, Jessica E. Burress, Purva S. Dalwadi, Kevin S. O’Fallon, Erin Gaffney-Stomberg et al.
    Pages 47-70

  6. Visualizing NETosis Using a Novel Neutrophil Extracellular Trap-Specific Marker

    • Meghan L. De Meo, Muhammad H. Shahzad, Jonathan D. Spicer
    Pages 71-80

  7. In Vitro Methods to Evaluate Macrophage Polarization and Function in Cancer

    • Aldo Ummarino, Clément Anfray, Akihiro Maeda, Fernando Torres Andón, Paola Allavena
    Pages 81-91

  8. In Vivo Analysis of Tumor-Associated Macrophages in the Tumor Microenvironment

    • Clément Anfray, Aldo Ummarino, Alfonso Calvo, Paola Allavena, Fernando Torres Andón
    Pages 93-108

  9. Assessing the Immune Regulatory Role of Metabolites In Vitro

    • Sarah McPhedran, Junu Choe, Sarah MacPherson, Gabrielle Kowalchuk, Vanessa Chan, Marisa K. Kilgour et al.
    Pages 109-120

  10. Mouse Models of Obesity to Study the Tumor-Immune Microenvironment

    • Sarah Petrecca, Daniela F. Quail
    Pages 121-138

  11. Generation and Quantification of Cytotoxic Lymphocytes Following Oncolytic Virus Infection of Multi-cellular Tumor Spheroids

    • Victoria A. Jennings, Carolina S. Ilkow
    Pages 139-149

  12. Assessing the Efficacy of Immune Checkpoint Inhibitors in Preclinical Tumor Models

    • Celia Jacoberger-Foissac, Bertrand Allard, David Allard, John Stagg
    Pages 151-169

  13. Fibroblast Isolation from Mammary Gland Tissue and Syngeneic Murine Breast Cancer Models

    • Margarita Bartish, Julian Smith-Voudouris, Sonia V. del Rincón
    Pages 171-185

  14. Multiscale Label-Free Imaging of Fibrillar Collagen in the Tumor Microenvironment

    • Michael S. Nelson, Yuming Liu, Helen M. Wilson, Bin Li, Ivan M. Rosado-Mendez, Jeremy D. Rogers et al.
    Pages 187-235

  15. In Situ Measurement of Intra-tumoral Tissue Rigidity

    • Benjamin E. Campbell, Stephanie Mok, Christopher Moraes
    Pages 237-246

  16. Extracellular Matrix Glycation and Crosslinking in Mammary Tumor Progression

    • Jason J. Northey, Valerie M. Weaver
    Pages 247-260

  17. Proteomic Assessment of the Murine Mammary Gland Extracellular Matrix

    • Samuel E. J. Preston, Vincent R. Richard, Sonia V. del Rincón, Christoph H. Borchers, René P. Zahedi
    Pages 261-271

  18. Spheroids as a 3D Model of the Hypoxic Tumor Microenvironment

    • Sarah M. Kirsh, Sydney A. Pascetta, James Uniacke
    Pages 273-285

  19. Tumor pH Imaging Using Chemical Exchange Saturation Transfer (CEST)-MRI

    • Dario Livio Longo, Elisa Pirotta, Riccardo Gambino, Feriel Romdhane, Antonella Carella, Alessia Corrado
    Pages 287-311

  20. Profiling the Epigenetic Landscape of the Tumor Microenvironment Using Chromatin Immunoprecipitation Sequencing

    • Marina Fukano, Gabriel Alzial, Raphaëlle Lambert, Geneviève Deblois
    Pages 313-348
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Keywords

About this book

This second edition provides update and new chapters detailing core and emerging in vitro and in vivo protocols.  Chapters guide readers through cellular and molecular biology approaches, in vivo genetic approaches, various “omics”-based strategies, therapeutic strategies, and advanced techniques in the fields of tissue engineering and nanotechnology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Cutting-edge and thorough, The Tumor Microenvironment: Methods and Protocols, Second Edition is a valuable resource for both novice and expert scientists in this developing field.

Editors and Affiliations

  • Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Canada

    Josie Ursini-Siegel

Bibliographic Information

  • Book Title: The Tumor Microenvironment

  • Book Subtitle: Methods and Protocols

  • Editors: Josie Ursini-Siegel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2914-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2913-0Published: 02 January 2023

  • Softcover ISBN: 978-1-0716-2916-1Published: 01 January 2023

  • eBook ISBN: 978-1-0716-2914-7Published: 01 January 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 414

  • Number of Illustrations: 15 b/w illustrations, 76 illustrations in colour

  • Topics: Cancer Research

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