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Protocols for Nucleic Acid Analysis by Nonradioactive Probes

  • Elena Hilario
  • John Mackay

Part of the Methods in Molecular Biology book series (MIMB, volume 353)

Table of contents

  1. Front Matter
    Pages i-x
  2. Nucleic Acid Extractions

    1. Front Matter
      Pages 1-1
    2. Elspeth MacRae
      Pages 15-24
  3. Indirect Detection

    1. Front Matter
      Pages 25-25
    2. Lisa S. Gellen, Glenn M. Wall-Manning, Chris H. Sissons
      Pages 39-67
    3. Christoph Peterhaensel, Dagmar Weier, Thomas Lahaye
      Pages 69-78
    4. Elena Hilario, Tiffany F. Bennell, Erik Rikkerink
      Pages 79-91
    5. Saeed A. Khan, Mohamed S. Nawaz
      Pages 93-103
    6. Shigeharu Moriya, Satoko Noda, Moriya Ohkuma, Toshiaki Kudo
      Pages 105-112
  4. Fluorescent Labeling and Detection

    1. Front Matter
      Pages 113-113
    2. María Rosa Ponce, Víctor Quesada, Andrea Hricová, José Luis Micol
      Pages 143-152
  5. Kinetic (“Real-Time”) PCR

  6. Microarrays

    1. Front Matter
      Pages 263-263
    2. Norman H. Lee, Alexander I. Saeed
      Pages 265-300
    3. Gaspar Taroncher-Oldenburg, Bess B. Ward
      Pages 301-315
  7. Back Matter
    Pages 317-321

About this book

Introduction

Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results.
The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Keywords

BAC DNA FISH Fluoreszenz in situ-Hybridisierung Microarray PCR Quantitative PCR Southern Blot biological materials gene expression genes hybridization molecular biology

Editors and affiliations

  • Elena Hilario
    • 1
  • John Mackay
    • 2
  1. 1.Gene TechnologiesHortResearch Ltd.Mt. Albert, AucklandNew Zealand
  2. 2.Applied ScienceRoche DiagnosticsAucklandNew Zealand

Bibliographic information

  • DOI https://doi.org/10.1385/1597452297
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-430-2
  • Online ISBN 978-1-59745-229-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site