Overview
- Editors:
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Kan Wang
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Center for Plant Transformation, Plant Science Institute, Iowa State Universtiy, Ames
Department of Agronomy, Iowa State Universtiy, Ames
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Table of contents (41 protocols)
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Tropic Plants
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- Juan B. Pérez, Serge Remy, Rony Swennen, László Sági
Pages 167-175
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- Vladimir Orbović, Jude W. Grosser
Pages 177-189
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- Thierry Leroy, Edgardo Alpizar, Magali Dufour, Hervé Etienne
Pages 191-208
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- Yun J. Zhu, Maureen M. M. Fitch, Paul H. Moore
Pages 209-217
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- Lourdes Yabor, Patricia Espinosa, Ariel D. Arencibia, José C. Lorenzo
Pages 219-226
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- Ariel D. Arencibia, Elva R. Carmona
Pages 227-235
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Nuts and Fruits
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Front Matter
Pages 237-237
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- Charles A. Maynard, Linda D. Polin, Sharon L. LaPierre, Ronald E. Rothrock, William A. Powell
Pages 239-251
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- Abhaya M. Dandekar, Gianni Teo, Sandra L. Uratsu, David Tricoli
Pages 253-261
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- Guo-Qing Song, Kenneth C. Sink
Pages 263-272
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- Alain Bouquet, Laurent Torregrosa, Pat Iocco, Mark R. Thomas
Pages 273-285
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- Bruno Mezzetti, Elisa Costantini
Pages 287-295
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- Charles A. Leslie, Sandra L. Uratsu, Gale McGranahan, Abhaya M. Dandekar
Pages 297-307
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Ornamental Plants
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Front Matter
Pages 309-309
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- Chalermsri Nontaswatsri, Seiichi Fukai
Pages 311-320
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- Jaime A. Teixeira da Silva
Pages 321-329
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- Ming-Tsair Chan, Yuan-Li Chan, Sanjaya
Pages 331-338
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- Schuyler S. Korban, Ksenija Gasic, Xiangqian Li
Pages 351-358
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Medicinal Plants
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Front Matter
Pages 359-359
About this book
Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.
Reviews
From the reviews of the second edition:
"This is, as the title suggests, a laboratory manual with protocols for using Agrobacterium tumefaciens to transform plant species … . the book will be a valuable addition to the laboratory bookshelf. The protocols … are clearly set out and the notes explain things … . I was also pleased that some chapters mentioned the need to consider regulatory issues regarding disposal of genetically modified materials, something that is often ignored in lab manuals." (Heather Macdonald, SGM – Society for General Microbiology, 2007)
Editors and Affiliations
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Center for Plant Transformation, Plant Science Institute, Iowa State Universtiy, Ames
Kan Wang
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Department of Agronomy, Iowa State Universtiy, Ames
Kan Wang