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Ion Channels

Methods and Protocols

  • James D. Stockand
  • Mark S. Shapiro

Part of the Methods in Molecular Biology™ book series (MIMB, volume 337)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Methods for Exogenous Expression of Ion Channels in Cells

    1. Front Matter
      Pages 1-38
    2. Alexander Staruschenko, Rachell E. Booth, Oleh Pochynyuk, James D. Stockand, Qiusheng Tong
      Pages 3-13
    3. Richard M. Ahlquist, Jane M. Sullivan
      Pages 15-26
  3. Methods for Studying Channel Structure-function

  4. Methods for Studying Channel Regulation and Physiological Function

    1. Front Matter
      Pages 79-153
    2. Bakhrom K. Berdiev, Dale J. Benos
      Pages 89-99
    3. Mouhamed S. Awayda, Weijian Shao, Ivana Vukojicic, Abderrahmane Bengrine
      Pages 101-115
  5. Methods for Studying Channelopathies, Genetic Screening, and Molecular Biology

    1. Front Matter
      Pages 155-205
    2. Gildas Loussouarn, Isabelle Baró, Denis Escande
      Pages 167-183
    3. Gildas Loussouarn, Isabelle Baró, Denis Escande
      Pages 185-205
  6. Back Matter
    Pages 207-212

About this book

Introduction

It is now clear that ion channels play essential roles in cell biology and physiology and that their dysfunction is the root cause of many human diseases. In Ion Channels: Methods and Protocols, internationally recognized biomedical scientists describe recent technological breakthroughs and demonstrate their use in successful experimental designs. The diverse applications range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, for studying channel regulation and physiological function, and for genetic screening and investigating channelopathies. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Ion Channels: Methods and Protocols offer both novice and experienced researchers a versatile collection of proven techniques for illuminating the structure, function, and regulation of ion channel proteins and finding suitable drug targets when they cause disease.

Keywords

Escherichia coli Lipid Vivo molecular biology quaternary structure regulation

Editors and affiliations

  • James D. Stockand
    • 1
  • Mark S. Shapiro
    • 1
  1. 1.University of Texas Health Science CenterSan AntonioUSA

Bibliographic information

  • DOI https://doi.org/10.1385/1597450952
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-576-7
  • Online ISBN 978-1-59745-095-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site