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Microchip Capillary Electrophoresis

Methods and Protocols

  • Charles S. Henry

Part of the Methods in Molecular Biology book series (MIMB, volume 339)

Table of contents

  1. Front Matter
    Pages i-x
  2. Microchip Capillary Electrophoresis

    1. Charles S. Henry
      Pages 1-9
  3. Microchip Fabrication Methods

    1. Front Matter
      Pages 11-46
    2. Mark M. Crain, Robert S. Keynton, Kevin M. Walsh, Thomas J. Roussel Jr., Richard P. Baldwin, John F. Naber et al.
      Pages 13-26
    3. Carlos D. García, Charles S. Henry
      Pages 27-35
    4. Laurie E. Locascio, David J. Ross, Peter B. Howell, Michael Gaitan
      Pages 37-46
  4. Surface Modification Methods

    1. Front Matter
      Pages 47-64
  5. Detection Methods for Microchip Capillary Electrophoresis

    1. Front Matter
      Pages 65-126
    2. Roland Hergenröder, Benedikt Graβ
      Pages 113-126
  6. Applications of Microchip Capillary Electrophoresis

    1. Front Matter
      Pages 127-231
    2. Andrea W. Chow
      Pages 129-143
    3. Andrea W. Chow
      Pages 145-158
    4. Barbara A. Fogarty, Nathan A. Lacher, Susan M. Lunte
      Pages 159-186
    5. S. Douglass Gilman, Peter J. Chapman
      Pages 187-201
    6. Christopher T. Culbertson
      Pages 203-216
    7. Christopher J Easley, Lindsay A. Legendre, James P. Landers, Jerome P. Ferrance
      Pages 217-231
  7. Back Matter
    Pages 233-237

About this book

Introduction

Microchip capillary electrophoresis, a new and emerging technology that can rapidly analyze biological compounds as small as or smaller than a single cell, will have a major impact on biological sciences in the next 10 to 20 years. In Microchip Capillary Electrophoresis: Methods and Protocols, leading chemists and engineers concisely explain the principles behind this revolutionary technique and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. The authors describe in detail the detection methods used, including mass spectrometry, electrochemistry, and conductivity. Since surface chemistry must be carefully considered for optimal operation at this scale, they also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Instructive and well-detailed, Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for both initiating research in the field of microchip capillary electrophoresis and understanding the full range of how these new systems can be used successfully in biological research.

Keywords

DNA Ion chemistry protein

Editors and affiliations

  • Charles S. Henry
    • 1
  1. 1.Colorado State UniversityFort CollinsUSA

Bibliographic information

  • DOI https://doi.org/10.1385/1597450766
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-293-3
  • Online ISBN 978-1-59745-076-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site