New and Emerging Proteomic Techniques

  • Dobrin Nedelkov
  • Randall W. Nelson

Part of the Methods in Molecular Biology™ book series (MIMB, volume 328)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Niroshan Ramachandran, Eugenie Hainsworth, Gokhan Demirkan, Joshua LaBaer
    Pages 1-14
  3. Brian B. Haab, Paul M. Lizardi
    Pages 15-29
  4. Bernhard H. Geierstanger, Petri Saviranta, Achim Brinker
    Pages 31-50
  5. Martijn Verdoes, Celia R. Berkers, Bogdan I. Florea, Paul F. van Swieten, Herman S. Overkleeft, Huib Ovaa
    Pages 51-69
  6. Terry T. Goodrich, Alastair W. Wark, Robert M. Corn, Hye Jin Lee
    Pages 113-130
  7. Dobrin Nedelkov, Randall W. Nelson
    Pages 131-139
  8. Urban A. Kiernan, Dobrin Nedelkov, Eric E. Niederkofler, Kemmons A. Tubbs, Randall W. Nelson
    Pages 141-150
  9. Christopher M. Colangelo, Kenneth R. Williams
    Pages 151-158
  10. Laurence Florens, Michael P. Washburn
    Pages 159-175
  11. Martin Ethier, Daniel Figeys, Hélène Perreault
    Pages 187-197
  12. Weichuan Yu, Baolin Wu, Junfeng Liu, Xiaoye Li, Kathy Stone, Kenneth R. Williams et al.
    Pages 199-216
  13. Back Matter
    Pages 229-232

About this book

Introduction

Numerous emerging technologies have propelled the growth of proteomics into a wide-ranging discipline with great potential to improve human health through the discovery of new protein biomarkers and therapeutics. In New and Emerging Proteomic Techniques, a panel of leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods described here span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. Where necessary, two or more distinct techniques are provided for a given problem, and advanced bioinformatics approaches are presented for the analysis of complex proteomic data. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and easy to use, New and Emerging Proteomic Techniques offers expert and novice investigators alike today's most promising proteomics techniques for probing the biological processes and understanding the diseases of the human body.

Keywords

Microarray Proteomics glycoprotein molecular biology proteins

Authors and affiliations

  • Dobrin Nedelkov
    • 1
  • Randall W. Nelson
    • 1
  1. 1.Intrinsic Bioprobes Inc.Tempe

Bibliographic information

  • DOI https://doi.org/10.1385/159745026X
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-519-4
  • Online ISBN 978-1-59745-026-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book