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In Situ Hybridization Protocols

  • Ian A. Darby
  • Tim D. Hewitson

Part of the Methods in Molecular Biology™ book series (MIMB, volume 326)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Gregory H. Tesch, Hui Y. Lan, David J. Nikolic-Paterson
    Pages 1-7
  3. Ian A. Darby, Teresa Bisucci, Alexis Desmoulière, Tim D. Hewitson
    Pages 17-31
  4. Ernst J. M. Speel, Anton H. N. Hopman, Paul Komminoth
    Pages 33-60
  5. Julia Asp, Alexandra Abramsson, Christer Betsholtz
    Pages 89-102
  6. Murray Hargrave, Josephine Bowles, Peter Koopman
    Pages 103-113
  7. Andrzej Loesch, Rainer Glass
    Pages 151-162
  8. Neil C. Owens, F. Martin Hess, Emilio Badoer
    Pages 163-171
  9. Changsun Choi, Laurie G. Hudson, Pierre Savagner, Donna F. Kusewitt
    Pages 173-188
  10. Daniel Benten, Kang Cheng, Sanjeev Gupta
    Pages 189-201
  11. Andreas Houben, Sharon J. Orford, Jeremy N. Timmis
    Pages 203-218
  12. Tim D. Hewitson, Kristen J. Kelynack, Ian A. Darby
    Pages 219-226
  13. Tim D. Hewitson, Teresa Bisucci, Ian A. Darby
    Pages 227-234
  14. Adrian M. Jubb, Thinh Q. Pham, Gretchen D. Frantz, Franklin V. Peale Jr., Kenneth J. Hillan
    Pages 255-264
  15. Back Matter
    Pages 265-269

About this book

Introduction

In In Situ Hybridization, Third Edition, Ian Darby updates his highly successful second edition and shifts the focus to tissue and cell in situ hybridization. Drawing on experts working in diverse areas and taking advantage of advances in non-isotopic in situ hybridization and amplification techniques, the authors describe in detail their readily reproducible techniques for preparation, detection, and tissue hybridization. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include the identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Completely updated and highly practical, In Situ Hybridization, Third Edition allows both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use these powerful new techniques successfully.

Keywords

DNA Microarray Termination electron microscopy gene expression genes hybridization molecular biology

Editors and affiliations

  • Ian A. Darby
    • 1
  • Tim D. Hewitson
    • 2
  1. 1.RMIT UniversityMelbourneAustralia
  2. 2.The Royal Melbourne Hospital and University of MelbourneMelbourneAustralia

Bibliographic information

  • DOI https://doi.org/10.1385/1597450073
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-402-9
  • Online ISBN 978-1-59745-007-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site