Cytochrome P450 Protocols

  • Ian R. Phillips
  • Elizabeth A. Shephard

Part of the Methods in Molecular Biology book series (MIMB, volume 320)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. David R. Nelson
    Pages 1-10
  3. John B. Schenkman, Ingela Jansson
    Pages 11-18
  4. Michael P. Pritchard, Lesley McLaughlin, Thomas Friedberg
    Pages 19-29
  5. F. Peter Guengerich, Martha V. Martin
    Pages 31-38
  6. Azara Janmohamed, Prapimpuk Thaunsukon, Elizabeth A. Shephard, Ian R. Phillips
    Pages 39-59
  7. Hiroshi Yamazaki, Tsutomu Shimada
    Pages 61-71
  8. Thomas K. H. Chang, David J. Waxman
    Pages 73-83
  9. David J. Waxman, Thomas K. H. Chang
    Pages 91-96
  10. Charles L. Crespi, Thomas K. H. Chang, David J. Waxman
    Pages 103-107
  11. Charles L. Crespi, Thomas K. H. Chang, David J. Waxman
    Pages 109-113
  12. Thomas K. H. Chang, Charles L. Crespi, David J. Waxman
    Pages 127-131

About this book

Introduction

For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Also described are techniques for the generation of mice with targeted gene disruptions. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source. Each chapter is written by researchers who have been involved in the development and application of the particular technique to P450s or FMOs. The protocols are presented in a step-by-step manner, with extensive cross-references to notes that highlight critical steps, potential problems, and alternative methods so that the researcher can understand the basis of the method and perform it successfully.
Cutting-edge and highly practical, Cytochrome P450, Second Edition provides both novices and experienced researchers across many fields all the tools needed today to elucidate the crucial biological role played by cytochrome P450 in the metabolism of therapeutic drugs, chemical carcinogens, and environmental pollutants.

Keywords

Allele Escherichia coli Oxidation Termination enzymes hybridization metabolism reproducible techniques

Editors and affiliations

  • Ian R. Phillips
    • 1
  • Elizabeth A. Shephard
    • 2
  1. 1.School of Biological SciencesQueen Mary University of LondonLondonUK
  2. 2.Department of Biochemistry and Molecular BiologyUniversity College LondonLondonUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592599982
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-441-8
  • Online ISBN 978-1-59259-998-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book