Differential Display Methods and Protocols

  • Peng Liang
  • Jonathan D. Meade
  • Arthur B. Pardee

Part of the Methods in Molecular Biology book series (MIMB, volume 317)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Methodologies

    1. Front Matter
      Pages 1-1
    2. Jonathan D. Meade, Yong-jig Cho, Jeffrey S. Fisher, Jamie C. Walden, Zhen Guo, Peng Liang
      Pages 23-57
    3. Mikhail V. Matz, Ella A. Meleshkevitch
      Pages 59-74
    4. Richard A. Shimkets
      Pages 75-83
    5. Dana M. Walters, Pierre E. Rouvière
      Pages 85-97
    6. Rosana Risques, Gaelle Rondeau, Martin Judex, Michael McClelland, John Welsh
      Pages 99-109
    7. Tero Aittokallio, Pekka Ojala, Timo J. Nevalainen, Olli S. Nevalainen
      Pages 111-122
    8. Ling Qin, Pjotr Prins, Johannes Helder
      Pages 123-138
  3. Applications

About this book

Introduction

Carrying on the high standards of the first edition of Differential Display Methods, Peng Liang et al. have based their second edition on a new mathematical model of differential display (DD) that takes advantage of automation, as well as digital data acquisition and analysis. These well-versed authors explain and highlight all the recent methodological refinements, including automated liquid handling of hundreds of DD PCR reaction setups combined with capillary electrophoresis, a prototype computer program to automatically allow positive band identification from a fluorescence differential display image, and restriction fragment-based DD screenings that can link any cDNA fragment directly to a given gene once the sequence information of all transcripts becomes available. Other improvements discussed are combining DD and DNA microarrays by reducing the complexity of cDNA probes while increasing the sensitivity of detection, and a DD approach to detect prokaryotic mRNA expression. The authors also demonstrate the power of DD technology with a collection of outstanding examples of DD applications and detailed experimental procedures. The elegant studies described here have led to the discovery of many important genes involved in viral infection, Prion disease, cancer, ovulation, circadian clock, floral color, transcription repression gene silencing, mRNA polymorphism, and protein-RNA interaction.
State-of-the-art and highly practical, Differential Display Methods, Second Edition offers gene hunters the possibility of genome-wide comprehensive DD screening, as well as a proven road map for any successful "gene fishing" expedition.

Keywords

DNA Microarray PCR fungi gene expression genes transcription

Editors and affiliations

  • Peng Liang
    • 1
  • Jonathan D. Meade
    • 2
  • Arthur B. Pardee
    • 3
  1. 1.Department of Cancer BiologyThe Vanderbilt-Ingram Cancer CenterNashville
  2. 2.GenHunter CorporationNashville
  3. 3.Dana Farber Cancer InstituteBoston

Bibliographic information

  • DOI https://doi.org/10.1385/1592599680
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-338-1
  • Online ISBN 978-1-59259-968-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book