Pharmacogenomics

Methods and Protocols

  • Federico Innocenti

Part of the Methods in Molecular Biology™ book series (MIMB, volume 311)

Table of contents

  1. Front Matter
    Pages i-x
  2. History and Overview

    1. Front Matter
      Pages 1-1
    2. Werner Kalow
      Pages 3-15
  3. Functional Analysis of Gene Variation

    1. Front Matter
      Pages 17-17
    2. Hideto Jinno, Nobumitsu Hanioka, Toshiko Tanaka-Kagawa, Yoshiro Saito, Jun-ichi Sawada, Shogo Ozawa
      Pages 19-29
    3. Julian Charles Knight
      Pages 49-60
  4. Genotyping Techniques

    1. Front Matter
      Pages 61-61
    2. Donna Lee Fackenthal, Pei Xian Chen, Soma Das
      Pages 73-96
    3. Sharon Marsh, Cristi R. King, Adam A. Garsa, Howard L. McLeod
      Pages 97-114
    4. Ian Craig, Emma Meaburn, Lee Butcher, Linzy Hill, Robert Plomin
      Pages 147-164
    5. Renato Robledo, William R. Beggs, Patrick K. Bender
      Pages 165-176
  5. Management of Pharmacogenomic Information

    1. Front Matter
      Pages 177-177
    2. Caroline F. Thorn, Teri E. Klein, Russ B. Altman
      Pages 179-191
    3. Alexander Sturn, Michael Maurer, Robert Molidor, Zlatko Trajanoski
      Pages 193-208

About this book

Introduction

Pharmacogenomics holds the promise of drugs tailor-made to an individual's genetic makeup for greater efficacy and safety. In Pharmacogenomics: Methods and Protocols, expert researchers and physician/clinicians describe in detail the newest and most common technologies now used in this rapidly advancing field. The authors provide readily reproducible methods for assessing the functional consequences of a certain polymorphism, evaluate the variety of genotyping platforms currently available, and discuss the management of pharmacogenomic information. Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. The methodologies for genotyping include denaturing high-performance liquid chromatography, pyrosequencing, kinetic-fluorescence detection, mass spectrometry, and TaqMan assay for insertion/deletions. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Pharmacogenomics: Methods and Protocols offers pharmacologists, geneticists, molecular biologists, and clinical chemists working in academia, biotechnology, and the pharmaceutical industry a cutting-edge collection of the key techniques used for today's preclinical and clinical studies.

Keywords

Allele DNA SNP gene expression genes genetics genomics microarray research

Editors and affiliations

  • Federico Innocenti
    • 1
  1. 1.Committee on Clinical Pharmacology and Pharmacogenomics, Section of Hematology/Oncology, Department of MedicineUniversity of ChicagoChicago

Bibliographic information

  • DOI https://doi.org/10.1385/1592599575
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-440-1
  • Online ISBN 978-1-59259-957-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book