Immunochemical Protocols

  • Robert Burns

Part of the Methods In Molecular Biology™ book series (MIMB, volume 295)

Table of contents

  1. Front Matter
    Pages i-x
  2. David C. Hancock, Nicola J. OReilly
    Pages 13-25
  3. David C. Hancock, Nicola J. OReilly
    Pages 27-39
  4. Robert Burns
    Pages 41-53
  5. Gary Entrican, Gareth Young
    Pages 55-70
  6. James D. Hirsch, Rosaria P. Haugland
    Pages 135-154
  7. David Hughes
    Pages 155-172
  8. David Hughes
    Pages 173-192
  9. William Jordan
    Pages 215-225
  10. Barbara Magi, Sabrina Liberatori
    Pages 227-253
  11. Glenn E. Morris
    Pages 255-267
  12. Weilin L. Shelver
    Pages 269-280

About this book

Introduction

This new third edition of a much praised classic has been updated and revised to provide both basic methods for producing and using antibodies and advanced techniques required by today's state-of-the art investigators. The basic sections of the book stress the first principles of antibody production and guide the reader through the processes and pitfalls of immunochemistry. The advanced sections focus on specific applications and cover the major immunochemical techniques currently used in laboratories. New material includes techniques for making new monoclonal cell lines, for producing good quality monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis. The authors provide full details of methods for antigen selection and preparation, antibody production, and reagent manufacture. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Up-to-date and highly practical, Immunochemical Protocols, Third Edition, takes both novice and experienced researchers from project initiation to final test format with a broad array of protocols suited to immediate use in the laboratory, as well as methods that can be successfully applied to entirely new systems and processes.

Keywords

PCR cell lines chemistry electron microscopy microscopy

Editors and affiliations

  • Robert Burns
    • 1
  1. 1.Antibody UnitScottish Agricultural Science AgencyEdinburghScotland

Bibliographic information

  • DOI https://doi.org/10.1385/1592598730
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-274-2
  • Online ISBN 978-1-59259-873-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book