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Cell Migration

Developmental Methods and Protocols

  • Jun-Lin Guan

Part of the Methods in Molecular Biology™ book series (MIMB, volume 294)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Donna J. Webb, Huaye Zhang, Alan F. Horwitz
      Pages 3-11
  3. Basic Cell Migration and Related Assays

    1. Front Matter
      Pages 13-13
    2. Hong-Chen Chen
      Pages 15-22
    3. Luis G. Rodriguez, Xiaoyang Wu, Jun-Lin Guan
      Pages 23-29
    4. Dennis F. Kucik, Chuanyue Wu
      Pages 43-54
    5. Allison L. Berrier, Susan E. LaFlamme
      Pages 55-68
    6. Hong-Chen Chen
      Pages 69-77
  4. Analysis of Cell Migration in Specialized Cell Systems

    1. Front Matter
      Pages 95-95
    2. Leslie M. Shaw
      Pages 97-105
    3. Chris Storgard, David Mikolon, Dwayne G. Stupack
      Pages 123-136
  5. Analysis of Cell Migration in Model Organisms

    1. Front Matter
      Pages 157-157
    2. M. Afaq Shakir, Erik A. Lundquist
      Pages 159-173
    3. Jocelyn A. McDonald, Denise J. Montell
      Pages 175-202
    4. William Wood, Antonio Jacinto
      Pages 203-210
    5. Diane S. Sepich, Lilianna Solnica-Krezel
      Pages 211-233
    6. Douglas W. DeSimone, Lance Davidson, Mungo Marsden, Dominique Alfandari
      Pages 235-245
    7. Maria Elena de Bellard, Marianne Bronner-Fraser
      Pages 247-267
    8. Mien V. Hoang, Donald R. Senger
      Pages 269-285
  6. Biochemical and Novel Approaches in Cell Migration

    1. Front Matter
      Pages 287-287
    2. Vidhya V. Iyer, Christoph Ballestrem, Joachim Kirchner, Benjamin Geiger, Michael D. Schaller
      Pages 289-302
    3. Frauke Drees, Amy Reilein, W. James Nelson
      Pages 303-320
    4. Andrea Flesken-Nikitin, Rebecca M. Williams, Warren R. Zipfel, Watt W. Webb, Alexander Yu. Nikitin
      Pages 335-345
    5. Daniel S. Rhoads, Sharvari M. Nadkarni, Loling Song, Camilla Voeltz, Eberhard Bodenschatz, Jun-Lin Guan
      Pages 347-357
  7. Back Matter
    Pages 359-364

About this book

Introduction

Central to the better understanding of both molecular mechanisms and disease, cell migration plays an essential role in a variety of biological processes and is now the subject of intense study using an array of powerful new technologies. In Cell Migration: Developmental Methods and Protocols, researchers describe in step-by-step detail their most successful techniques for studying the macromolecular machinery of cell movement. These readily reproducible protocols include a wide range of novel and state-of-the-art methodologies, as well as many classic methods, for use in cultured cells, different model organisms, and specialized cells in both normal development and disease. Highlights include basic assays that apply to all cell migration studies in vitro, assays in various model organisms, and assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. The authors also offer several novel approaches to the study of cell migration, as well as extensive coverage of cell migration studies in developmental and disease models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Cell Migration: Developmental Methods and Protocols offers researchers easy access to many readily reproducible techniques for the optimally productive investigation of cell migration in today's interdisciplinary experimental environment.

Keywords

Drosophila Vivo biology cancer cell development membrane microscopy migration molecular biology protein

Editors and affiliations

  • Jun-Lin Guan
    • 1
  1. 1.The Department of Molecular Medicine, College of Veterinary MedicineCornell UniversityIthaca

Bibliographic information

  • DOI https://doi.org/10.1385/1592598609
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-382-4
  • Online ISBN 978-1-59259-860-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site