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Laser Capture Microdissection

Methods and Protocols

  • Book
  • © 2005

Overview

Editors:
  1. Graeme I. Murray

    1. Department of Pathology, University of Aberdeen, Aberdeen, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Stephanie Curran

    1. Department of Pathology, University of Aberdeen, Aberdeen, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 293)

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Table of contents (24 protocols)

  1. RNA and Gene Expression Studies Using Microdissected Cells

    1. Laser Capture Microdissection for Analysis of Macrophage Gene Expression From Atherosclerotic Lesions

      • Eugene Trogan, Edward A. Fisher
      Pages 221-232

    2. Analysis of Pituitary Cells by Laser Capture Microdissection

      • Ricardo V. Lloyd, Xiang Qian, Long Jin, Katharina Ruebel, Jill Bayliss, Shuya Zhang et al.
      Pages 233-241

  2. Microdissection Techniques And Applications In Proteomics

    1. Front Matter

      Pages 243-243
      Download chapter PDF

    2. Laser Capture Microdissection and Colorectal Cancer Proteomics

      • Laura C. Lawrie, Stephanie Curran
      Pages 245-254

    3. Proteomic Analysis of Human Bladder Tissue Using SELDI® Approach Following Microdissection Techniques

      • Rene C. Krieg, Nadine T. Gaisa, Cloud P. Paweletz, Ruth Knuechel
      Pages 255-267

  3. Microdissection And Molecular Analysis Of Microorganisms

    1. Front Matter

      Pages 269-269
      Download chapter PDF

    2. Genetic Analysis of HIV by In Situ PCR-Directed Laser Capture Microscopy of Infected Cells

      • Daniele Marras
      Pages 271-284

    3. Use of Laser Capture Microdissection Together With In Situ Hybridization and Real-Time PCR to Study Distribution of Latent Herpes Simplex Virus Genomes in Mouse Trigeminal Ganglia

      • Xiao-Ping Chen, Marina Mata, David J. Fink
      Pages 285-293

    4. Laser Capture Microdissection and PCR for Analysis of Human Papilloma Virus Infection

      • Kheng Chew, Patrick H. Rooney, Margaret E. Cruickshank, Graeme I. Murray
      Pages 295-300

    5. Laser Capture Microdissection of Hepatic Stages of the Human Parasite Plasmodium falciparum for Molecular Analysis

      • Jean-Philippe Semblat, Olivier Silvie, Jean-François Franetich, Dominique Mazier
      Pages 301-307

  4. Back Matter

    Pages 309-319
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Keywords

About this book

Expert laboratorians noted for their optimized methods describe in step-by-step detail how to successfully carry out tissue-based laser microdissection in their own laboratory using the different systems available and to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. Highlights include gene expression profiling of primary tumor cell populations using laser capture microdissection, RNA transcript amplification and GeneChip® microarrays, laser capture microdissection and colorectal cancer proteomics, and proteomic analysis of human bladder tissue using SELDI® approach following microdissection techniques. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics.

Reviews

From the reviews:

"...an excellent help to researchers...." - Neoplasma

"Comprehensive and state of the art, this book provides a highly practical guide to successful tissue-based laser microdissection using available commercial systems, and also a guide to interpreting the results using a wide range of sophisticated molecular technologies." - Clinical Laboratory International

"...provides researchers with practical advise and detailed technical protocols for carrying out tissue-based LCM...." - Vet Pathol

"Comprehensive and up-to-date collection of readily reproducible preparative and analytical methods is presented in each chapter for an in-depth practice of laser microdissection application. … The book is subdivided into 5 parts with a total of 24 chapters featuring step-by-step protocols that will enable novice researchers to isolate specific cell types or microorganisms." (Carlo Alberto Redi, European Journal of Histochemistry, December, 2005)

Editors and Affiliations

  • Department of Pathology, University of Aberdeen, Aberdeen, UK

    Graeme I. Murray, Stephanie Curran

Bibliographic Information

  • Book Title: Laser Capture Microdissection

  • Book Subtitle: Methods and Protocols

  • Editors: Graeme I. Murray, Stephanie Curran

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592598536

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • eBook ISBN: 978-1-59259-853-3Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 320

  • Number of Illustrations: 81 b/w illustrations, 1 illustrations in colour

  • Topics: Biochemistry, general, Cell Biology

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