DNA Viruses

Methods and Protocols

  • Paul M. Lieberman

Part of the Methods in Molecular Biology book series (MIMB, volume 292)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Viral Detection

  3. Virus Structure And Imaging

    1. Front Matter
      Pages 83-171
    2. Alexander J. Malkin, Marco Plomp, Alexander McPherson
      Pages 85-108
    3. Vitaly Erukhimovitch, Marina Talyshinsky, Yelena Souprun, Mahmoud Huleihel
      Pages 161-172
  4. Virus Entry

    1. Front Matter
      Pages 173-211
    2. Hirofumi Sawa, Rika Komagome
      Pages 175-185
    3. Marisa P. McShane, Richard Longnecker
      Pages 187-195
  5. Gene Expression

    1. Front Matter
      Pages 213-244
    2. Brian A. Van Tine, Thomas R. Broker, Louise T. Chow
      Pages 215-230

About this book

Introduction

Recently, modern laboratory techniques have been applied with great success to DNA viruses, an important viral class that shares basic survival strategies and mechanisms of pathogenesis, and also provides model systems for the investigation of basic biological processes. In DNA Viruses: Methods and Protocols, a panel of expert academic and biomedical researchers describe in detail the innovative methods they have developed to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in research virology with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.

Keywords

DNA PCR Termination cell lines electron microscopy gene expression genes transcription

Editors and affiliations

  • Paul M. Lieberman
    • 1
  1. 1.The Wistar InstitutePhiladelphia

Bibliographic information

  • DOI https://doi.org/10.1385/159259848X
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-353-4
  • Online ISBN 978-1-59259-848-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book