Phosphodiesterase Methods and Protocols

  • Claire Lugnier

Part of the Methods In Molecular Biology™ book series (MIMB, volume 307)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Marco Mongillo, Anna Terrin, Sandrine Evellin, Valentina Lissandron, Manuela Zaccolo
    Pages 1-13
  3. Akira Honda, Carolyn L. Sawyer, Sharon M. Cawley, Wolfgang R. G. Dostmann
    Pages 27-43
  4. Thomas C. Rich, Jeffrey W. Karpen
    Pages 45-61
  5. Thérèse Keravis, Rima Thaseldar-Roumié, Claire Lugnier
    Pages 63-74
  6. Faiyaz Ahmad, Linda Härndahl, Yan Tang, Lena Stenson Holst, Vincent C. Manganiello
    Pages 93-107
  7. Hanguan Liu, Jing Rong Tang, Eva Degerman, Vincent C. Manganiello
    Pages 109-124
  8. Dana C. Pentia, Suzanne Hosier, Rachel A. Collupy, Beverly A. Valeriani, Rick H. Cote
    Pages 125-140
  9. Wito Richter, Thomas Hermsdorf, Dietrich Dettmer
    Pages 155-165
  10. Hengming Ke, Qing Huai, Robert X. Xu
    Pages 181-190
  11. S.-L. Catherine Jin, Anne M. Latour, Marco Conti
    Pages 191-210
  12. James L. Weeks II, Mitsi A. Blount, Alfreda Beasley, Roya Zoraghi, Melissa K. Thomas, Konjeti Raja Sekhar et al.
    Pages 239-262
  13. Khakim G. Muradov, Kimberly K. Boyd, Nikolai O. Artemyev
    Pages 263-275

About this book

Introduction

The multigenic family of cyclic nucleotide phosphodiesterases (PDEs) is now known to offer many new therapeutic possibilities through their influence over intracellular signaling, though their precise cell mechanisms to modulating that process remain uncertain. In Phosphodiesterase Methods and Protocols, a panel of research leaders in the PDE field describes new concepts and techniques for investigating the role of PDEs in orchestrating normal and pathophysiological responses. Presented in step-by-step detail, these readily reproducible methods allow the measurement of cyclic nucleotide variations in living cells, as well as their visualization in a spatiotemporal manner, the localization and characterization of their activities in tissues and living cells, and the assessment of targeted PDEs in creating specific tools and drugs. Additional chapters discuss the generation of PDE4 knockout mice to demonstrate not only the potential role of targeted PDEs, but also their use in further studies of the central role of PDE regulation in intracellular signaling control. These techniques offer clinicians a way to find new therapies for numerous pathologies whose molecular origins are unknown and whose treatment is symptomatic. Alterations of intracellular signaling related to PDE deregulation in such pathologies as inflammation, neurodegeneration, and cancer may explain the difficulties observed in their prevention and treatment. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Path-breaking and highly practical, Phosphodiesterase Methods and Protocols offers cell biologists, molecular biologists, pharmacologists, and medicinal chemists a broad ranging survey of the advanced tools and concepts needed to understand the role of PDEs in physiological functions, their implications in several critical pathologies, and the opportunities they offer for new drug design.

Keywords

Calcium Escherichia coli Filtration Lipid Nucleotide Promoter Termination genes hybridization

Editors and affiliations

  • Claire Lugnier
    • 1
  1. 1.Pharmacolgoie et Physico-Chimie des Interactions Celluaires et Moléculaires, Centre National de la Recherche ScientifiqueUniversité Louis Pasteur de Strasbourg, Faculté de PharmacieIllkirchFrance

Bibliographic information

  • DOI https://doi.org/10.1385/1592598390
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-314-5
  • Online ISBN 978-1-59259-839-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book