Stroke Genomics

1. Front Matter

   Pages i-x

   PDF

2. Introduction

   1. Front Matter

      Pages 1-1

      PDF

   2. Introduction to Stroke Genomics

      • Simon J. Read, Frank C. Barone

      Pages 3-15

3. Preclinical Models and Gene Manipulation

   1. Front Matter

      Pages 17-17

      PDF

   2. Choice, Methodology, and Characterization of Focal Ischemic Stroke Models

      • David Virley

      Pages 19-48

   3. Mutant Animal Models of Stroke and Gene Expression

      • Hilary V. O. Carswell, Martin W. McBride, Delyth Graham, Anna F. Dominiczak, I. Mhairi Macrae

      Pages 49-74

   4. Gene Therapy in Neurological Disease

      • Midori A. Yenari, Robert M. Sapolsky

      Pages 75-87

   5. Stem Cell Transplantation After Middle Cerebral Artery Occlusion

      • Paul Stroemer, Helen Hodges

      Pages 89-104

   6. Endogenous Brain Protection

      • Frank C. Barone

      Pages 105-184

   7. Production of Transgenic and Mutant Mouse Models

      • Alex J. Harper

      Pages 185-202

   8. 3-Nitropropionic Acid Model of Metabolic Stress

      • Toby John Roberts

      Pages 203-220

4. Clinical Paradigms

   1. Front Matter

      Pages 221-221

      PDF

   2. Practicalities of Genetic Studies in Human Stroke

      • Ahamad Hassan, Hugh S. Markus

      Pages 223-240

   3. Evaluation of the Interactions of Common Genetic Mutations in Stroke

      • Zoltán Szolnoki

      Pages 241-249

5. Assessing Differential Expression

   1. Front Matter

      Pages 251-251

      PDF

   2. Technologies of Disease-Related Gene Discovery Using Preclinical Models of Stroke

      • Xinkang Wang

      Pages 253-264

   3. Quantitative Analysis of Gene Transcription in Stroke Models Using Real-Time RT-PCR

      • David C. Harrison, Brian C. Bond

      Pages 265-283

   4. Effective Analysis of Genomic Data

      • Paul R. Nelson, Andrew B. Goulter, Richard J. Davis

      Pages 285-311

   5. Bioinformatic Approaches to Assigning Protein Function From Novel Sequence Data

      • David Michalovich, Richard Fagan

      Pages 313-332

   6. Pragmatic Target Discovery From Novel Gene to Functionally Defined Drug Target

      • Stuart McRae Allan

      Pages 333-346

With sequencing of the human genome now complete, deciphering the role of gene function in human neurological pathophysiology is a promise that has yet to be realized. More than most diseases, stroke has been keenly studied from a genomic perspective. Studies are numerous and incorporate data on stroke inheritance, chromosomal loci of risk, preclinical models of stroke, and differential gene expression of brain injury, repair, and recovery. The problem is no longer a lack of information but one of interpretation and prioritization of what we do know. The aims of Stroke Genomics: Methods and Reviews are twofold. First, it aims to provide the reader with cutting-edge reviews of clinical and preclinical genomics, written by leading experts in the field. In particular, the authors of certain chapters relate gene expression changes to physiological end points, such as functional imaging paradigms. Thus, a more holistic approach to gene expression is described, one in which molecular biology goes hand in hand with stroke pathophysiology. Second, detailed methods for study of the molecular biology of stroke are ™ also included. Following the format of the Methods in Molecular Medicine series, these chapters will enable the reader to employ each technique without recourse to other methods texts. In its entirety, this book should provide the reader with the knowledge needed to design, execute, and interpret preclinical and clinical studies of stroke genomics.

• Chromosom
• PCR
• gene expression
• gene therapy
• genes
• physiology
• transcription

From the reviews:

"The protocols presented in Stroke Genomics: Methods and Reviews will greatly benefit scientists interested in the molecular biology as well as the genetic and genomic basis of strokes." - Journal of Chemical Neuroanatomy

"...very interesting and informative...an attractive and interesting overview of the different aspects of the current stroke research...can be recommended as a source of useful and up-to-date practical and theoretical information on stroke genetics and on stroke associated gene expression." - European Neurology

"This book gives the opportunity for experts in the field to explore the practical and experimental applications of genomic and molecular biology research to the treatment of stroke. … The book also indicates detailed methods for studying the molecular biology of stroke. … It can be recommended if one is interested in the basic scientific background that underlies central nervous system pathology … ." (J. V. Byrne, Neuroradiology, Vol. 47, 2005)

• AstraZeneca Pharmaceuticals Macclesfield, Cheshire, UK

  Simon J. Read

• Neurology Centre for Excellence in Drug Discovery GlaxoSmithKline Pharmaceuticals, Harlow, UK

  David Virley

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