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Oligonucleotide Synthesis

  • Piet Herdewijn

Part of the Methods in Molecular Biology book series (MIMB, volume 288)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Douglas J. Dellinger, Jason R. Betley, Tadeusz K. Wyrzykiewicz, Marvin H. Caruthers
    Pages 1-16
  3. Stephanie A. Hartsel, David E. Kitchen, Stephen A. Scaringe, William S. Marshall
    Pages 33-49
  4. Zhiwei Wang, Quanlai Song, Yogesh S. Sanghvi
    Pages 51-63
  5. Annie Galarneau, Kyung-Lyum Min, Maria M. Mangos, Masad J. Damha
    Pages 65-80
  6. Jacek Stawinski, Roger Ströberg
    Pages 81-100
  7. Enrique Pedroso, Nuria Escaja, Miriam Frieden, Anna Grandas
    Pages 101-125
  8. Henrik M. Pfundheller, Anders M. Sørensen, Christian Lomholt, Anders M. Johansen, Troels Koch, Jesper Wengel
    Pages 127-145
  9. Vladimir A. Efimov, Oksana G. Chakhmakhcheva
    Pages 147-163
  10. Frank Seela, Yang He, Junlin He, Georg Becher, Rita Kröschel, Matthias Zulauf et al.
    Pages 165-186
  11. Darrell R. Davis, Ashok C. Bajji
    Pages 187-204
  12. Dmitry A. Stetsenko, Michael J. Gait
    Pages 205-224
  13. A. Michael Morocho, Valeri Karamyshev, Olga Shcherbinina, Nikolai Polushin
    Pages 225-240
  14. Ali Laayoun, Eloy Bernal-Méndez, Isabelle Sothier, Mitsuharu Kotera, Alain Troesch
    Pages 241-250
  15. Alexandre S. Boutorine, Jian-Sheng Sun
    Pages 251-260
  16. Jacqueline A. M. Vet, Salvatore A. E. Marras
    Pages 273-290
  17. Nikolai Polushin, Andrei Malykh, A. Michael Morocho, Alexei Slesarev, Sergei Kozyavkin
    Pages 291-304
  18. Marcus Hey, Michael Göel
    Pages 305-318
  19. Christian Bailly, Jérôme Kluza, Christopher Martin, Thomas Ellis, Michael J. Waring
    Pages 319-342
  20. Peter E. Nielsen
    Pages 343-358
  21. Hani S. Zaher, Peter J. Unrau
    Pages 359-378
  22. Eric Dausse, Christian Cazenave, Bernard Rayner, Jean-Jacques Toulmé
    Pages 391-409
  23. Jean-Rémi Bertrand, Andrei Maksimenko, Claude Malvy
    Pages 411-429
  24. Back Matter
    Pages 431-435

About this book

Introduction

Nucleic acids chemistry has progressed rapidly in the last 10 years with the introduction of many new techniques and the development of many novel therapeutic entities. In Oligonucleotide Synthesis: Methods and Applications, laboratory experts describe in step-by-step detail the powerful new techniques they have developed for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are the synthesis of phosphorothioates via new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, triple helix formation, and a novel two-step process that yields a high purity DNA that is less costly. Novel applications include the use of molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules to DNA, nucleic acid libraries, optimization of RNA synthesis for siRNA applications, and the use of small interference RNA (siRNA) as an inhibitor of gene expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Oligonucleotide Synthesis: Methods and Applications offers today's investigators not only insight into important new biotechnologies, but also a full range of the detailed protocols needed to work successfully in DNA synthesis and its applications today.

Keywords

DNA PCR gene expression genes siRNA

Editors and affiliations

  • Piet Herdewijn
    • 1
  1. 1.Rega Institute, Medicinal Chemistry LabKatholieke Universiteit LeuvenLeuvenBelgium

Bibliographic information

  • DOI https://doi.org/10.1385/1592598234
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-233-9
  • Online ISBN 978-1-59259-823-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site