Cell Cycle Control and Dysregulation Protocols

Cyclins, Cyclin-Dependent Kinases, and Other Factors

  • Antonio Giordano
  • Gaetano Romano

Part of the Methods in Molecular Biology™ book series (MIMB, volume 285)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Analysis of Cyclins and Cyclin-Dependent Kinases

    1. Front Matter
      Pages 1-1
    2. Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 23-28
    3. Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 29-31
    4. Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 33-36
    5. Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 37-40
    6. Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 41-44
    7. Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 45-55
    8. Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 57-62
    9. Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan et al.
      Pages 63-67
    10. Adrian M. Senderowicz
      Pages 69-78
    11. Henry Hoff, Hong Zhang, Christian Sell
      Pages 79-92
  3. Analysis of the Factors Involved in Cell Cycle Deregulation

    1. Front Matter
      Pages 93-93
    2. Henry Hoff, Barbara Belletti, Hong Zhang, Christian Sell
      Pages 95-104
    3. Martyn K. White, Caterina Cinti
      Pages 105-111
    4. Carmela Trimarchi, Dario La Sala, Alessandra Zamparelli, Caterina Cinti
      Pages 113-118
  4. Cellular Response to Deoxyribonucleic Acid Damage

    1. Front Matter
      Pages 119-119
    2. Salvatore Cortellino, David P. Turner, Domenico Albino, Alfonso Bellacosa
      Pages 121-125
    3. Salvatore Cortellino, David P. Turner, Alfonso Bellacosa
      Pages 127-132

About this book

Introduction

The dysregulation of the normal cell cycle underlies an enormous number of pathological conditions, including the neurodegenerative disorders, all cancers, and diabetes. In Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors, expert laboratorians detail emerging methodologies for studying the cell cycle, its kinases, and kinase inhibitors. The authors focus on issues of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Many of the protocols are new, such as electron microsocopy to detect apoptosis and proteosome-mediated degradation, whereas others study the interactions between protein-protein, protein-DNA, and protein-RNA. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors offers pathbreaking researchers powerful tools for probing the cell cycle regulation across a wide variety of pathologies.

Editors and affiliations

  • Antonio Giordano
    • 1
  • Gaetano Romano
    • 2
  1. 1.Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and TechnologyTemple UniversityPhiladelphia
  2. 2.Department of NeurosurgeryThomas Jefferson UniversityPhiladelphia

Bibliographic information

  • DOI https://doi.org/10.1385/1592598226
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-949-0
  • Online ISBN 978-1-59259-822-9
  • Series Print ISSN 1064-3745
  • About this book