Platelets and Megakaryocytes

Volume 2: Perspectives and Techniques

  • Jonathan M. Gibbins
  • Martyn P. Mahaut-Smith

Part of the Methods in Molecular Biology™ book series (MIMB, volume 273)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Perspectives

    1. Front Matter
      Pages 1-1
    2. Donna Woulfe, Jing Yang, Nicolas Prevost, Peter O’Brien, Ryan Fortna, Massimiliano Tognolini et al.
      Pages 3-31
    3. Nikla R. Emambokus, George J. Murphy, Jonathan Frampton
      Pages 33-55
    4. José Rivera, María L. Lozano, Vicente Vicente
      Pages 57-72
    5. Chris J. Vlahos, Louis F. Stancato
      Pages 87-102
  3. Signaling Techniques

    1. Front Matter
      Pages 103-103
    2. Stephanie M. Jung, Masaaki Moroi
      Pages 105-114
    3. Dermot Cox
      Pages 125-137
    4. Matthew L. Jones, Alastair W. Poole
      Pages 169-177
    5. Gertie Gorter, Jan Willem Akkerman
      Pages 179-199
    6. Bernard Payrastre
      Pages 201-212
    7. Corie N. Shrimpton, Karine Gousset, Fern Tablin, José A. López
      Pages 213-228
    8. Philippe Ohlmann, Béatrice Hechler, Jean-Pierre Cazenave, Christian Gachet
      Pages 229-250

About this book

Introduction

Platelets are an essential element of the body's hemostasis system, and yet, through their involvement in thrombosis, are also a major cause of morbidity and mortality. Platelets have a limited lifespan and must be continually manufactured by their precursor, the megakaryocyte. In Platelets and Megakaryocytes, Volume 2: Perspectives and Techniques leading expert researchers-many responsible for seminal discoveries in the field-offer basic and advanced techniques for studying cell signaling in the regulation and function of platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts in the regulation of cell signaling, and the measurement of intracellular calcium levels. There are also techniques for the study of electrophysiological events, nitric oxide signaling, venom proteins, and for the internalization of proteins into platelets through permeabilization. The application of many molecular and post-genomic methods to the study of platelets and megakaryocytes is also described. All protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Platelets and Megakaryocytes, Volume 1: Functional Assays describes in step-by-step detail basic and advanced techniques for analyzing platelet and megakaryocyte function.
Comprehensive and cutting-edge, the two volumes of Platelets and Megakaryocytes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.

Keywords

Activation Proteomics Viruses Western Blotting cell lines gene expression genes microscopy molecular biology platelets proteins

Editors and affiliations

  • Jonathan M. Gibbins
    • 1
  • Martyn P. Mahaut-Smith
    • 2
  1. 1.School of Animal and Microbial SciencesThe University of ReadingReadingUK
  2. 2.Department of PhysiologyUniversity of CambridgeCambridgeUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592597831
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-011-3
  • Online ISBN 978-1-59259-783-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book