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Genetic Recombination

Reviews and Protocols

  • Alan S. Waldman

Part of the Methods in Molecular Biology™ book series (MIMB, volume 262)

Table of contents

  1. Front Matter
    Pages i-x
  2. Studying Recombination Events in Eukaryotes

    1. Front Matter
      Pages 1-1
    2. Waltraud Schmidt-Puchta, Nadiya Orel, Anzhela Kyryk, Holger Puchta
      Pages 25-34
    3. Richard McCulloch, Erik Vassella, Peter Burton, Michael Boshart, J. David Barry
      Pages 53-86
    4. Laura Reinholdt, Terry Ashley, John Schimenti, Naoko Shima
      Pages 87-107
  3. Recombination as a Reporter of Genomic Instability

    1. Front Matter
      Pages 109-109
    2. Richard J. Brennan, Robert H. Schiestl
      Pages 111-124
    3. Ramune Reliene, Alexander J. R. Bishop, Jiri Aubrecht, Robert H. Schiestl
      Pages 125-139
  4. Recombination as a Tool for Producing Targeted Genetic Modification

    1. Front Matter
      Pages 141-141
    2. Glenn M. Manthey, Michelle S. Navarro, Adam M. Bailis
      Pages 157-172
    3. Jean Y. Kuan, Peter M. Glazer
      Pages 173-194
  5. Biochemistry of Recombination

    1. Front Matter
      Pages 221-221
    2. Angelos Constantinou, Stephen C. West
      Pages 239-253
  6. Back Matter
    Pages 255-260

About this book

Introduction

Genetic recombination plays an essential role in such vitally important biological processes as DNA repair, regulation of gene expression, disease etiology, meiotic chromosome segregation, and evolution. In Genetic Recombination: Reviews and Protocols, prominent international investigators actively engaged in recombination research describe in great detail their best techniques for studying recombination. The methods range from approaches and model systems to be used in a variety of eukaryotic organisms and in a mammalian parasite, to biochemical analyses useful for furthering the understanding of recombination mechanisms. Additional techniques use recombination as a reporter of genomic instability in lower and higher eukaryotes and as a tool for producing targeted genetic modification. Each readily reproducible method includes step-by-step instructions, a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Genetic Recombination: Reviews and Protocols offers both novice and experienced investigators an illuminating synthesis of what we currently know about recombination, as well as a wide-ranging collection of powerful tools for elucidating its mechanisms and regulation throughout biology today.

Editors and affiliations

  • Alan S. Waldman
    • 1
  1. 1.Department of Biological SciencesUniversity of South CarolinaCoumbia

Bibliographic information

  • DOI https://doi.org/10.1385/1592597610
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-236-0
  • Online ISBN 978-1-59259-761-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site