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Transcription Factor Protocols

  • Martin J. Tymms

Part of the Methods in Molecular Biology™ book series (MIMB, volume 130)

Table of contents

  1. Front Matter
    Pages i-x
  2. Dennis K. Watson, Richard Kitching, Calvin Vary, Ismail Kola, Arun Seth
    Pages 1-11
  3. Gerd P. Pfeifer, Stella Tommasi
    Pages 13-27
  4. Hans-Henrik M. Dahl, Wendy M. Hutchison
    Pages 47-57
  5. Kurt Gustin, Robert D. Burk
    Pages 85-90
  6. Elaine T. Schenborn
    Pages 91-102
  7. Elaine T. Schenborn, Virginia Goiffon
    Pages 135-145
  8. Elaine T. Schenborn, Virginia Goiffon
    Pages 147-153
  9. Elaine T. Schenborn, Jennifer Oler
    Pages 155-164
  10. S. Roy Himes, M. Frances Shannon
    Pages 165-174
  11. Peter L. Molloy
    Pages 235-246
  12. Dan S. Prestridge
    Pages 265-295
  13. Back Matter
    Pages 297-306

About this book

Introduction

In Transcription Factor Protocols, Martin Tymms has created a powerful compendium of the major techniques for the study of those DNA sequences and protein factors that regulate the transcription of protein encoding genes. With chapters contributed by leading investigators well versed in the methods, this eminently practical compendium includes not only well established protocols, but also novel techniques that are now being widely adopted. Among the important new methods treated are the use of triplex-forming oligonucleotides, the application of whole genome PCR to the isolation of gene promoters/enhancers, the analysis of in vivo methylation, and in vivo footprinting using UV light and ligation-mediated PCR. Step-by-step instructions, tips about pitfalls to avoid, and extensive procedural notes all help to ensure robust and reproducible results.Transcription Factor Protocols offers both experienced workers and new researchers a vital first-stop reference collection of the core techniques for all those exploring the role of transcription factors in gene regulation today.

Editors and affiliations

  • Martin J. Tymms
    • 1
  1. 1.National Vision Research Institute of AustraliaMelbourneAustralia

Bibliographic information

  • DOI https://doi.org/10.1385/159259686X
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-573-7
  • Online ISBN 978-1-59259-686-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site