Overview
- Editors:
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Peter B. Becker
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Adolf-Butenandt-Institut-Molekularbiologie, Ludwig-Maximilians-Universität, München, Germany
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Table of contents (36 protocols)
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- Karolin Luger, Thomas J. Rechsteiner, Timothy J. Richmond
Pages 1-16
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- David R. Chafin, Kyu-Min Lee, Jeffrey J. Hayes
Pages 27-43
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- Andrew Flaus, Timothy J. Richmond
Pages 45-60
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- Ariel Prunell, Mohamed Alilat, Filomena De Lucia
Pages 79-101
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- Simon Chandler, Alan P. Wolffe
Pages 103-112
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- Jeffrey C. Hansen, Terace M. Fletcher, J. Isabelle Kreider
Pages 113-125
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- Jeffrey C. Hansen, Cynthia L. Turgeon
Pages 127-141
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- Sanford H. Leuba, Carlos Bustamante
Pages 143-160
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- Ralf Erik Wellinger, Renzo Lucchini, Reinhard Dammann, José M. Sogo
Pages 161-173
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- Edgar Bonte, Peter B. Becker
Pages 187-194
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- Raphael Sandaltzopoulos, Peter B. Becker
Pages 195-206
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- Wladyslaw A. Krajewski, Peter B. Becker
Pages 207-217
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- Anne-Elisabeth de la Barre, Michel Robert-Nicoud, Stefan Dimitrov
Pages 219-229
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- Pierre-Henri Gaillard, Danièle Roche, Geneviève Almouzni
Pages 231-243
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- Magdalena Livingstone-Zatchej, Bernhard Suter, Fritz Thoma
Pages 245-259
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About this book
More than 40 years after the discovery of the nucleosome as the fun- mental unit of chromatin, the multifaceted problem of how variations in ch- matin structure affect the activity of the eukaryotic genome has not been solved. However, during the past few years research on chromatin structure and fu- tion has gained considerable momentum, and impressive progress has been made at the level of concept development as well as filling in crucial detail. The structure of the nucleosome has been visualized at unprecedented reso- tion. Powerful multisubunit enzymes have been identified that alter histone/ DNA interactions in ways that expose regulatory sequences to factors initi- ing and regulating such nuclear processes as transcription. Though the imp- tance of posttranslational modifications of histones, notably their acetylation, has long been known, the finding that a number of bona fide regulators increase transcription by acetylating nucleosomes has lent new support to the old idea that the process of gene regulation is intimately related to the nature of the chromatin environment. A wealth of nonhistone proteins contribute to a continuum of structures with distinct biochemical properties and varying degrees of DNA condensation. Perhaps the most important conclusion from a large number of studies is a fresh appreciation of the dynamic nature of chromatin structure, the built-in flexibility providing the basis for regulation.
Reviews
"Laboratories working with chromatin or concerned with mammalian gene expression in context will profit from the focus in this volume."-Doody's Health Science Book Review Journal
"Chromatin Protocols represents an exhaustive collection (over 500 pages) of back-ground information and protocols to investigate various aspects of chromatin structure and function....The breadth of the topics covered makes Chromatin Protocols appealing to most cell biologists and a must for specialists in the field....the range and richness of the protocols treated make this volume a valuable reference...Your lab should definitely get it and your library should get it, as the book will certainly remain up to date in the years to come."-Cell Biology International
Editors and Affiliations
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Adolf-Butenandt-Institut-Molekularbiologie, Ludwig-Maximilians-Universität, München, Germany
Peter B. Becker
