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DNA Repair Protocols

Eukaryotic Systems

  • Daryl S. Henderson

Part of the Methods in Molecular Biology™ book series (MIMB, volume 113)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Mutant Isolation and Gene Cloning

  3. Recognition and Removal of Inappropriate or Damaged DNA Bases

    1. Front Matter
      Pages 101-101
    2. Byung Joon Hwang, Vaughn Smider, Gilbert Chu
      Pages 103-120
    3. Stephanie E. Corrette-Bennett, Robert S. Lahue
      Pages 121-132
    4. Shirley McCready
      Pages 147-156
    5. Betsy M. Sutherland, Paula V. Bennett, John C. Sutherland
      Pages 183-202
    6. Günter Speit, Andreas Hartmann
      Pages 203-212
    7. Keith A. Grimaldi, John P. Bingham, John A. Hartley
      Pages 227-240
    8. Keith A. Grimaldi, Simon R. McAdam, John A. Hartley
      Pages 241-255
    9. R. Michael Anson, Vilhelm A. Bohr
      Pages 257-279
    10. Guido Frosina, Enrico Cappelli, Paola Fortini, Eugenia Dogliotti
      Pages 301-315
    11. Johnson M. S. Wong, Zhigang He, C. James Ingles
      Pages 317-326
    12. Bernard Salles, Patrick Calsou
      Pages 327-335
    13. Eric J. Ackerman, Lilia K. Koriazova, Jitendra K. Saxena, Alexander Y. Spoonde
      Pages 347-355
    14. Mahmud K. K. Shivji, Jonathan G. Moggs, Isao Kuraoka, Richard D. Wood
      Pages 373-392
  4. DNA Strand Breakage and Repair

    1. Front Matter
      Pages 393-393
    2. Allyson Holmes, James E. Haber
      Pages 403-415
    3. Gregory B. Gloor, Tammy Dray, Kathy Keeler
      Pages 425-438
    4. Vladic A. Mogila, Yohanns Bellaiche, Norbert Perrimon
      Pages 439-445
    5. George Iliakis, Nge Cheong
      Pages 473-485
    6. Feng Liang, Maria Jasin
      Pages 487-497
  5. DNA Damage Tolerance Mechanisms and Regulatory Responses

    1. Front Matter
      Pages 527-527
    2. Andrew T. M. Vaughan, Scott Walter, Anne E. Milner
      Pages 519-526
    3. John Cunniff, Justin Blethrow, William Sullivan
      Pages 527-533
    4. Nicolaas G. J. Jaspers, Malgorzata Z. Zdzienicka
      Pages 535-542

About this book

Introduction

In DNA Repair Protocols: Eukaryotic Systems, Daryl S. Henderson and a team of hands-on experts give time-tested instructions for analyzing a wide range of DNA repair processes and cellular responses to DNA damage, including nucleotide and base excision repair, DNA strand break repair, and mismatch repair. The methods focus on eukaryotic model systems that have made, or have the potential to make, important contributions to our understanding of cellular responses to DNA damage in relation to mutagenesis, carcinogenesis, and the cell cycle. Although mammalian cells predominate, consideration is also given to such important nonmammalian model systems as yeast, C. elegans (nematode), Drosophila (fruitfly), Xenopus (amphibian), and plants.

DNA Repair Protocols: Eukaryotic Systems offers the most comprehensive collection of DNA repair protocols available, all step-by-step, optimized, and eminently reproducible with tips on troubleshooting and avoiding pitfalls. It will serve as the gold-standard reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches.

Keywords

DNA Ligation PCR Quantitative PCR cell lines genes saccharomyces cerevisiae

Editors and affiliations

  • Daryl S. Henderson
    • 1
  1. 1.University of DundeeDundeeUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592596754
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-802-8
  • Online ISBN 978-1-59259-675-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site