MAP Kinase Signaling Protocols

  • Rony Seger

Part of the Methods in Molecular Biology™ book series (MIMB, volume 250)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Hadara Rubinfeld, Rony Seger
    Pages 1-28
  3. Sarah Kraus, Rony Seger
    Pages 29-47
  4. Rafael Pulido, Ángel Zúñiga, Axel Ullrich
    Pages 49-60
  5. John M. Kyriakis, Hong Liu, Deborah N. Chadee
    Pages 61-88
  6. Richard I. Tapping, Kato Yutaka, Ta-Hsiang Chao, Masaaki Hayashi, Jeng-Fan Lo, Sung-Woo Kim et al.
    Pages 89-96
  7. Josema Torres, Carmen Blanco-Aparicio, Rafael Pulido
    Pages 103-112
  8. Yoav D. Shaul, Rony Seger
    Pages 113-125
  9. Elizabeth J. Goldsmith, Melanie H. Cobb, Chung-I Chang
    Pages 127-143
  10. Makoto Adachi, Eisuke Nishida
    Pages 145-153
  11. Gilles Pagès, Jacques Pouysségur
    Pages 155-166
  12. Baltazar D. Aguda, Herbert M. Sauro
    Pages 167-175
  13. Bose S. Kochupurakkal, Yosef Yarden
    Pages 177-202
  14. Piero Crespo, J. Silvio Gutkind
    Pages 203-210
  15. Karine R. Bernard, Karen R. Jonscher, Katheryn A. Resing, Natalie G. Ahn
    Pages 263-281
  16. Andrew N. Hoofnagle, Katheryn A. Resing, Natalie G. Ahn
    Pages 283-298

About this book

Introduction

Mitogen-activated protein kinase (MAPK) signaling cascades initiate highly significant cellular processes and are involved in a large number of diseases, including cancer and diabetes. In MAP Kinase Signaling Protocols, leading researchers with extensive hands-on experience describe in detail both classic and cutting-edge techniques for the detection and measurement of the MAPKs and other components in these cascades. The protocols include methods for the determination of the subcellular localization of these components, the structural and biophysical analysis of the components, and identification of novel components of known and unknown signaling cascades. Additional methods examine the upstream mechanisms of activation of MAPK cascades by various receptors, the mechanisms involved in the downregulation of the MAPK cascades, and identification of targets of the MAPK cascades. A number of techniques use inhibitors in research on MAPK and transgenic mice for studies of MAPK signaling. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and versatile, MAP Kinase Signaling Protocols offers biochemists, cell biologists, physicians, and biotechnologists all the essential laboratory techniques needed to conduct productive studies of MAPK signaling in health and disease, in measuring the influence of drugs, and in a broad range of experimental systems.

Editors and affiliations

  • Rony Seger
    • 1
  1. 1.Department of Biological RegulationThe Zeizmann Institute of ScienceRehovotIsrael

Bibliographic information

  • DOI https://doi.org/10.1385/1592596711
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-998-8
  • Online ISBN 978-1-59259-671-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book