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  • © 2004

Atomic Force Microscopy

Biomedical Methods and Applications

Part of the book series: Methods in Molecular Biology (MIMB, volume 242)

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  • ISBN: 978-1-59259-647-8
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Table of contents (28 protocols)

  1. Subcellular Structures Investigation

    1. Atomic Force Microscopy of Interfacial Monomolecular Films of Pulmonary Surfactant

      • Kaushik Nag, Robert R. Harbottle, Amiyo K. Panda, Nils O. Petersen
      Pages 231-243
    2. High-Resolution Analysis of the 3D Organization of Human Metaphase Chromosomes

      • Stefan Thalhammer, Pietro Gobbi, Mirella Falconi, Giovanni Mazzotti, Wolfgang M. Heckl
      Pages 245-254
    3. Shape and Volume of Living Aldosterone-Sensitive Cells Imaged with the Atomic Force Microscope

      • Stefan W. Schneider, Rainer Matzke, Manfred Radmacher, Hans Oberleithner
      Pages 255-279
    4. Localization of Epithelial Sodium Channels by Atomic Force Microscopy

      • Peter R. Smith, Dale J. Benos
      Pages 281-289
    5. High-Resolution Imaging of Bacteriorhodopsin by Atomic Force Microscopy

      • Dimitrios Fotiadis, Andreas Engel
      Pages 291-303
  2. Functional Investigations with AFM

    1. Front Matter

      Pages 305-305
    2. Observation of Oxidative Stress on Yeast Cells

      • Ricardo de Souza Pereira
      Pages 315-321
    3. Lymphoblastoid Cells Exposed to Low-Frequency Magnetic Fields

      • Settimio Grimaldi, Marco Girasole, Antonio Cricenti
      Pages 323-339
    4. Atomic Force Microscopy of β-Amyloid

      • Justin Legleiter, Tomasz Kowalewski
      Pages 349-364
    5. Measurement of Single Molecular Interactions by Dynamic Force Microscopy

      • Martin Hegner, Wilfried Grange, Patricia Bertoncini
      Pages 369-381
  3. Back Matter

    Pages 383-394

About this book

Although atomic force microscopy (AFM) offers many significant advantages over the conventional microscopies used in the biological and medical sciences, its use is more familiar to physicists and engineers than to biomedical researchers. In Atomic Force Microscopy: Biomedical Methods and Applications, highly experienced physicians and biologists clearly explain the basic technical knowledge needed to use AFM and demonstrate its multifarious uses in biomedicine and the life sciences. The applications range widely from morphostructural analyses of cellular structures, to the investigation of subcellular structures, to functional investigations, and reveal a powerful new way of looking at biological samples. Each tested protocol includes step-by-step instructions to ensure successful experimental results, background material on the principle behind the technique, tips on troubleshooting and avoiding known pitfalls, and notes on how to distinguish artifacts from useful data. The methods clearly demonstrate the advantages of AFM over traditional life science microscopy, among them simultaneous very high magnification and resolution, minimal tissue and cell preparation, and the ability to obtain different views of the sample from a single data collection.
Cutting-edge and highly practical, Atomic Force Microscopy: Biomedical Methods and Applications will help all investigators in biology and medicine open a new microscopic world, develop novel applications, and apply this powerful technology productively in their own work.

Editors and Affiliations

  • Department of Pharmacology School of Medicine, University of Milan, Milan, Italy

    Pier Carlo Braga

  • Department of Biophysical and Electronic Engineering, University of Genoa, Genoa, Italy

    Davide Ricci

Bibliographic Information

  • Book Title: Atomic Force Microscopy

  • Book Subtitle: Biomedical Methods and Applications

  • Editors: Pier Carlo Braga, Davide Ricci

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592596479

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2004

  • Hardcover ISBN: 978-1-58829-094-6

  • Softcover ISBN: 978-1-4899-3931-9

  • eBook ISBN: 978-1-59259-647-8

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 394

  • Topics: Biochemistry

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-59259-647-8
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 149.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)