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  • Book
  • © 2004

Atomic Force Microscopy

Biomedical Methods and Applications

Editors:

  1. Pier Carlo Braga

    1. Department of Pharmacology School of Medicine, University of Milan, Milan, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Davide Ricci

    1. Department of Biophysical and Electronic Engineering, University of Genoa, Genoa, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 242)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. The Basics of Atomic Force Microscopy

    1. Front Matter

      Pages 1-1
      PDF

    2. How the Atomic Force Microscope Works

      • Davide Ricci, Pier Carlo Braga
      Pages 3-12

    3. Imaging Methods in Atomic Force Microscopy

      • Davide Ricci, Pier Carlo Braga
      Pages 13-23

    4. Recognizing and Avoiding Artifacts in AFM Imaging

      • Davide Ricci, Pier Carlo Braga
      Pages 25-37

    5. Advanced Biosensing Using Micromechanical Cantilever Arrays

      • Martin Hegner, Youri Arntz
      Pages 39-49

  3. Morphostructural Analysis of Cellular Structures

    1. Front Matter

      Pages 51-51
      PDF

    2. Analysis of Human Fibroblasts by Atomic Force Microscopy

      • Gillian R. Bushell, Colm Cahill, Sverre Myhra, Gregory S. Watson
      Pages 53-67

    3. Corneal Tissue Observed by Atomic Force Microscopy

      • Stylliani Lydataki, Miltiadis K. Tsilimbaris, Eric S. Lesniewska, Alain Bron, Iannis G. Pallikaris
      Pages 69-83

    6. Imaging Living Chondrocyte Surface Structures With AFM Contact Mode

      • Gerlinde Bischoff, Anke Bernstein, David Wohlrab, Hans-Joachim Hein
      Pages 105-124

    7. Growth Cones of Living Neurons Probed by Atomic Force Microscopy

      • Davide Ricci, Massimo Grattarola, Mariateresa Tedesco
      Pages 125-140

    8. Evaluating Demineralization and Mechanical Properties of Human Dentin With AFM

      • Grayson W. Marshall Jr., Sally J. Marshall, Mehdi Balooch, John H. Kinney
      Pages 141-159

    9. Applying Atomic Force Microscopy to Studies in Cardiac Physiology

      • Jason J. Davis, Trevor Powell, H. Allen O. Hill
      Pages 161-178

  4. Subcellular Structures Investigation

    1. Front Matter

      Pages 189-189
      PDF

    2. Visualizing Nuclear Structure In Situ by Atomic Force Microscopy

      • Luis Felipe Jiménez-García, María de Lourdes Segura-Valdez
      Pages 191-199

    4. Atomic Force Microscopy of Protein Complexes

      • Olga I. Kiselyova, Igor V. Yaminsky
      Pages 217-230
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About this book

The natural, biological, medical, and related sciences would not be what they are today without the microscope. After the introduction of the optical microscope, a second breakthrough in morphostructural surface analysis occurred in the 1940s with the development of the scanning electron microscope (SEM), which, instead of light (i. e. , photons) and glass lenses, uses electrons and electromagnetic lenses (magnetic coils). Optical and scanning (or transmission) electron microscopes are called “far-field microscopes” because of the long distance between the sample and the point at which the image is obtained in comparison with the wavelengths of the photons or electrons involved. In this case, the image is a diffraction pattern and its resolution is wavelength limited. In 1986, a completely new type of microscopy was proposed, which, without the use of lenses, photons, or electrons, directly explores the sample surface by means of mechanical scanning, thus opening up unexpected possibilities for the morphostructural and mechanical analysis of biological specimens. These new scanning probe microscopes are based on the concept of near-field microscopy, which overcomes the problem of the limited diffraction-related resolution inherent in conventional microscopes. Located in the immediate vicinity of the sample itself (usually within a few nanometers), the probe records the intensity, rather than the interference signal, thus significantly improving resolution. Since the most we- known microscopes of this type operate using atomic forces, they are frequently referred to as atomic force microscopes (AFMs).
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Editors and Affiliations

  • Department of Pharmacology School of Medicine, University of Milan, Milan, Italy

    Pier Carlo Braga

  • Department of Biophysical and Electronic Engineering, University of Genoa, Genoa, Italy

    Davide Ricci

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Bibliographic Information

  • Book Title: Atomic Force Microscopy

  • Book Subtitle: Biomedical Methods and Applications

  • Editors: Pier Carlo Braga, Davide Ricci

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592596479

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2004

  • Hardcover ISBN: 978-1-58829-094-6Published: 02 October 2003

  • Softcover ISBN: 978-1-4899-3931-9Published: 11 August 2013

  • eBook ISBN: 978-1-59259-647-8Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 394

  • Topics: Biochemistry, general

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Buy it now

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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