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  • © 2004

Atomic Force Microscopy

Biomedical Methods and Applications

Part of the book series: Methods in Molecular Biology (MIMB, volume 242)

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  • ISBN: 978-1-59259-647-8
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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiv
  2. The Basics of Atomic Force Microscopy

    1. Front Matter

      Pages 1-1
    2. How the Atomic Force Microscope Works

      • Davide Ricci, Pier Carlo Braga
      Pages 3-12
    3. Imaging Methods in Atomic Force Microscopy

      • Davide Ricci, Pier Carlo Braga
      Pages 13-23
    4. Recognizing and Avoiding Artifacts in AFM Imaging

      • Davide Ricci, Pier Carlo Braga
      Pages 25-37
    5. Advanced Biosensing Using Micromechanical Cantilever Arrays

      • Martin Hegner, Youri Arntz
      Pages 39-49
  3. Morphostructural Analysis of Cellular Structures

    1. Front Matter

      Pages 51-51
    2. Analysis of Human Fibroblasts by Atomic Force Microscopy

      • Gillian R. Bushell, Colm Cahill, Sverre Myhra, Gregory S. Watson
      Pages 53-67
    3. Corneal Tissue Observed by Atomic Force Microscopy

      • Stylliani Lydataki, Miltiadis K. Tsilimbaris, Eric S. Lesniewska, Alain Bron, Iannis G. Pallikaris
      Pages 69-83
    4. Imaging Living Chondrocyte Surface Structures With AFM Contact Mode

      • Gerlinde Bischoff, Anke Bernstein, David Wohlrab, Hans-Joachim Hein
      Pages 105-124
    5. Growth Cones of Living Neurons Probed by Atomic Force Microscopy

      • Davide Ricci, Massimo Grattarola, Mariateresa Tedesco
      Pages 125-140
    6. Evaluating Demineralization and Mechanical Properties of Human Dentin With AFM

      • Grayson W. Marshall Jr., Sally J. Marshall, Mehdi Balooch, John H. Kinney
      Pages 141-159
    7. Applying Atomic Force Microscopy to Studies in Cardiac Physiology

      • Jason J. Davis, Trevor Powell, H. Allen O. Hill
      Pages 161-178
  4. Subcellular Structures Investigation

    1. Front Matter

      Pages 189-189
    2. Visualizing Nuclear Structure In Situ by Atomic Force Microscopy

      • Luis Felipe Jiménez-García, María de Lourdes Segura-Valdez
      Pages 191-199
    3. Atomic Force Microscopy of Protein Complexes

      • Olga I. Kiselyova, Igor V. Yaminsky
      Pages 217-230

About this book

Although atomic force microscopy (AFM) offers many significant advantages over the conventional microscopies used in the biological and medical sciences, its use is more familiar to physicists and engineers than to biomedical researchers. In Atomic Force Microscopy: Biomedical Methods and Applications, highly experienced physicians and biologists clearly explain the basic technical knowledge needed to use AFM and demonstrate its multifarious uses in biomedicine and the life sciences. The applications range widely from morphostructural analyses of cellular structures, to the investigation of subcellular structures, to functional investigations, and reveal a powerful new way of looking at biological samples. Each tested protocol includes step-by-step instructions to ensure successful experimental results, background material on the principle behind the technique, tips on troubleshooting and avoiding known pitfalls, and notes on how to distinguish artifacts from useful data. The methods clearly demonstrate the advantages of AFM over traditional life science microscopy, among them simultaneous very high magnification and resolution, minimal tissue and cell preparation, and the ability to obtain different views of the sample from a single data collection.
Cutting-edge and highly practical, Atomic Force Microscopy: Biomedical Methods and Applications will help all investigators in biology and medicine open a new microscopic world, develop novel applications, and apply this powerful technology productively in their own work.

Editors and Affiliations

  • Department of Pharmacology School of Medicine, University of Milan, Milan, Italy

    Pier Carlo Braga

  • Department of Biophysical and Electronic Engineering, University of Genoa, Genoa, Italy

    Davide Ricci

Bibliographic Information

  • Book Title: Atomic Force Microscopy

  • Book Subtitle: Biomedical Methods and Applications

  • Editors: Pier Carlo Braga, Davide Ricci

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592596479

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2004

  • Hardcover ISBN: 978-1-58829-094-6

  • Softcover ISBN: 978-1-4899-3931-9

  • eBook ISBN: 978-1-59259-647-8

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 394

  • Topics: Biochemistry

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-59259-647-8
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 149.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)