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Membrane Protein Protocols

Expression, Purification, and Characterization

  • Book
  • © 2003

Overview

Editors:
  1. Barry S. Selinsky

    1. Department of Chemistry, Villanova University, Villanova

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Part of the book series: Methods in Molecular Biology (MIMB, volume 228)

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Table of contents (25 protocols)

  1. Membrane Protein Purification

    1. Purification, Reconstitution, and Functional Characterization of Zinc Transporter from Rat Renal Brush Border Membranes

      • Rajendra Prasad
      Pages 195-221

    2. Isolation of Lipid Raft-Associated Proteolipids

      • Jaime Millán, María C. de Marco, Mohammed Qaidi, Alicia Batista, Fernando Martín-Belmonte, Miguel A. Alonso
      Pages 223-230

    3. Purification of Membrane-Bound Catechol-O-Methyltransferase

      • Maria João Bonifácio, Patrício Soares-da-Silva
      Pages 231-238

    4. Purification and Characterization of Transporter Proteins from Human Erythrocyte Membrane

      • Da-Neng Wang, M. Joanne Lemieux, Jonathan M. Boulter
      Pages 239-255

    5. Purification of the Human Erythrocyte PS-Stimulated Mg2+-ATPase

      • Jill V. Lyles, Kathleen Cornely-Moss, Christine M. Smith, David L. Daleke
      Pages 257-269

    6. Reconstitution and Assay of Biogenic Membrane-Derived Phospholipid Flippase Activity in Proteoliposomes

      • Sathyanarayana N. Gummadi, Sigrún Hrafnsdóttir, Jane Walent, William E. Watkins, Anant K. Menon
      Pages 271-279

    7. Expression, Purification, and Reconstitution of Rat Liver Carnitine Palmitoyltransferase I

      • Nicholas F. Brown
      Pages 281-301

  2. Structural Analysis of Membrane Proteins

    1. Front Matter

      Pages 303-303
      Download chapter PDF

    2. Crystallization in Lipidic Cubic Phases

      • Valentin I. Gordeliy, Ramona Schlesinger, Rouslan Efremov, Georg Büldt, Joachim Heberle
      Pages 305-316

    3. Optical Biosensor Assay Using Retroviral Receptor Pseudotypes

      • Joseph Rucker
      Pages 317-328

  3. Back Matter

    Pages 329-334
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About this book

Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.

Reviews

"Investigators working with membrane proteins will want to acquire this book. This is a useful compilation of protocols. . .there is much of utility in this book"-Doody's Health Sciences Book Review Journal

"...a useful reference work for researchers investigating the structure and function of membrane proteins." -- Immunology News

"...provides good food for thought for new and advanced membrane protein researchers." - ChemBioChem

"...highly recommendable to people working in proteomics..." - Proteomics Journal

Editors and Affiliations

  • Department of Chemistry, Villanova University, Villanova

    Barry S. Selinsky

Bibliographic Information

  • Book Title: Membrane Protein Protocols

  • Book Subtitle: Expression, Purification, and Characterization

  • Editors: Barry S. Selinsky

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/159259400X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2003

  • Hardcover ISBN: 978-1-58829-124-0Published: 29 May 2003

  • Softcover ISBN: 978-1-61737-376-3Published: 10 November 2010

  • eBook ISBN: 978-1-59259-400-9Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXII, 334

  • Topics: Biochemistry, general

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