Overview
- Editors:
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Barry S. Selinsky
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Department of Chemistry, Villanova University, Villanova
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Table of contents (25 protocols)
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Membrane Protein Purification
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- Jaime Millán, MarÃa C. de Marco, Mohammed Qaidi, Alicia Batista, Fernando MartÃn-Belmonte, Miguel A. Alonso
Pages 223-230
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- Maria João Bonifácio, PatrÃcio Soares-da-Silva
Pages 231-238
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- Da-Neng Wang, M. Joanne Lemieux, Jonathan M. Boulter
Pages 239-255
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- Jill V. Lyles, Kathleen Cornely-Moss, Christine M. Smith, David L. Daleke
Pages 257-269
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- Sathyanarayana N. Gummadi, Sigrún Hrafnsdóttir, Jane Walent, William E. Watkins, Anant K. Menon
Pages 271-279
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Structural Analysis of Membrane Proteins
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Front Matter
Pages 303-303
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- Valentin I. Gordeliy, Ramona Schlesinger, Rouslan Efremov, Georg Büldt, Joachim Heberle
Pages 305-316
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Back Matter
Pages 329-334
About this book
Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.
Reviews
"Investigators working with membrane proteins will want to acquire this book. This is a useful compilation of protocols. . .there is much of utility in this book"-Doody's Health Sciences Book Review Journal
"...a useful reference work for researchers investigating the structure and function of membrane proteins." -- Immunology News
"...provides good food for thought for new and advanced membrane protein researchers." - ChemBioChem
"...highly recommendable to people working in proteomics..." - Proteomics Journal
Editors and Affiliations
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Department of Chemistry, Villanova University, Villanova
Barry S. Selinsky