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PCR Protocols

  • John M. S. Bartlett
  • David Stirling

Part of the Methods in Molecular Biology™ book series (MIMB, volume 226)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Introduction to PCR

    1. Front Matter
      Pages 1-1
    2. John M. S. Bartlett, David Stirling
      Pages 3-6
    3. Peter Carroll, David Casimir
      Pages 7-14
    4. Susan McDonagh
      Pages 15-19
    5. David Stirling
      Pages 21-23
  3. Preparation of Nucleic Acid Templates

    1. Front Matter
      Pages 25-25
    2. John M. S. Bartlett
      Pages 27-28
    3. John M. S. Bartlett, Anne White
      Pages 29-31
    4. Helen Pearson, David Stirling
      Pages 33-34
    5. Helen Pearson
      Pages 43-44
    6. John M. S. Bartlett
      Pages 45-46
    7. Helen Pearson
      Pages 47-48
    8. David Stirling, John M. S. Bartlett
      Pages 49-51
    9. Wera M. Schmerer
      Pages 57-61
    10. David Stirling
      Pages 63-63
    11. John M. S. Bartlett
      Pages 65-75
  4. Basic PCR Methods

    1. Front Matter
      Pages 79-79
    2. David L. Hyndman, Masato Mitsuhashi
      Pages 81-88
    3. Haiying Grunenwald
      Pages 89-99
    4. Xin Wang, W. Scott Young III
      Pages 105-115
    5. Ranil S. Dassanayake, Lakshman P. Samaranayake
      Pages 117-122
    6. Marie A. Iannone, J. David Taylor, Jingwen Chen, May-Sung Li, Fei Ye, Michael P. Weiner
      Pages 123-133
    7. William H. Benjamin Jr., Kim R. Smith, Ken B. Waites
      Pages 135-149
    8. Antonio Olmos, Olga Esteban, Edson Bertolini, Mariano Cambra
      Pages 151-159
    9. Kenji Abe
      Pages 161-166
    10. Raymond Tellier, Jens Bukh, Suzanne U. Emerson, Robert H. Purcell
      Pages 167-172
    11. Raymond Tellier, Jens Bukh, Suzanne U. Emerson, Robert H. Purcell
      Pages 173-177
  5. Ultrasensitive and Quantitative PCR

    1. Front Matter
      Pages 179-179
    2. David Stirling
      Pages 181-183
    3. Friedrich W. Cremer, Marion Moos
      Pages 185-196
    4. John M. S. Bartlett
      Pages 205-210
    5. Ron Kerr
      Pages 211-214
  6. Transcriptome Analysis

    1. Front Matter
      Pages 215-215
    2. John M. S. Bartlett
      Pages 217-223
    3. Orlando Dominguez, Lidia Sabater, Yaqoub Ashhab, Eva Belloso, Ricardo Pujol-Borrell
      Pages 225-236
    4. Kostya Khalturin, Sergej Kuznetsov, Thomas C. G. Bosch
      Pages 237-243
    5. Steven Ringquist, Gaelle Rondeau, Rosa-Ana Risques, Takuya Higashiyama, Yi-Peng Wang, Steffen Porwollik et al.
      Pages 245-253
    6. Stephen Case-Green, Clare Pritchard, Edwin Southern
      Pages 255-269
    7. Karin A. Oien
      Pages 271-283
  7. Mutations and Polymorphisms

About this book

Introduction

Drawing on the proven qualities of the much praised and widely used first edition, John M. S. Bartlett and David Stirling have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These successful methods include real-time PCR, SNP analysis, nested PCR, direct PCR, and long-range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. In situ PCR methods and their application in parallel with other methods, such as immunohistochemistry, are also included. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
Cutting-edge and highly practical, PCR Protocols, Second Edition provides both novice and experienced investigators with an up-to-date compendium of powerful PCR methods for easy reference and consultation in the day-to-day performance of PCR-based experimentation, one that will enhance understanding of PCR, satisfy current needs, and point to powerful future applications.

Keywords

DNA Microarray PCR Polymerase Chain Reaction Quantitative PCR SNP Single Nucleotide Polymorphism Viruses biological materials fungi genes tissue

Editors and affiliations

  • John M. S. Bartlett
    • 1
  • David Stirling
    • 2
  1. 1.Department of SurgeryGlasgow Royal InfirmaryGlasgowUK
  2. 2.Department of HematologyRoyal Infirmary of EdinburghEdinburghUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592593844
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-642-0
  • Online ISBN 978-1-59259-384-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site