Generation of cDNA Libraries

Methods and Protocols

  • Shao-Yao Ying

Part of the Methods in Molecular Biology™ book series (MIMB, volume 221)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Shao-Yao Ying
    Pages 1-12
  3. Yue Zhang
    Pages 13-24
  4. Zhaohui Wang, Michael G. K. Jones
    Pages 25-31
  5. Mikhail V. Matz, Naila O. Alieva, Alex Chenchik, Sergey Lukyanov
    Pages 41-49
  6. Sheng-He Huang, Steven H. M. Chen, Ambrose Y. Jong
    Pages 51-58
  7. Hans-Jürgen Fülle
    Pages 145-153
  8. Igor Nepluev, Rodney J. Folz
    Pages 161-168
  9. San Ming Wang, Scott C. Fears, Lin Zhang, Jian-Jun Chen, Janet D. Rowley
    Pages 197-205
  10. Jian-Jun Chen, Sanggyu Lee, Guolin Zhou, Janet D. Rowley, San Ming Wang
    Pages 207-222

About this book

Introduction

With the decoding of the human genome and the potential application of its information to gene chips, the ability to generate cDNA libraries for functional assays of specific gene sequences has become critical in gene manipulation and genetic research. In Generation of cDNA Libraries: Methods and Protocols, expert researchers and inventors in the field describe their own proven techniques for generating cDNA/mRNA libraries to identify the functions of specific decoded gene sequences. A wide variety of techniques is presented for enhancing the generation of complete and full-length libraries, and for confirming the quality of the cDNAs generated. Among the applications detailed are electrophoresis, Northern blotting, single-cell microarray analysis, subtractive hybridization, subtractive cloning, gene cloning, and peptide library generation. Each method includes background information, step-by-step instructions, a list of reagents, operational tips, and notes on instrumentation. A background tutorial provides essential information: the definition of a cDNA library, the various types of cDNA libraries, the differences between methods for cDNA library generation using either conventional approaches or novel strategies, and the quality of cDNA libraries.
State-of-the-art and highly practical, Generation of cDNA Libraries: Methods and Protocols provides a variety of powerful and readily reproducible techniques for the generation of the entire range of complete, full-length cDNA/mRNA libraries needed in today's forefront genetic research.

Editors and affiliations

  • Shao-Yao Ying
    • 1
  1. 1.Keck School of MedicineUniversity of Southern CaliforniaLos Angeles

Bibliographic information

  • DOI https://doi.org/10.1385/1592593593
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-066-3
  • Online ISBN 978-1-59259-359-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book