Overview
- Editors:
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John Q. Wang
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School of Pharmacy, University of Missouri-Kansas City, Kansas, USA
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Table of contents (35 protocols)
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Detection of Protein Expression in the Striatum
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- Steffany A. L. Bennett, David C. S. Roberts
Pages 283-295
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Gene Function Analysis
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Front Matter
Pages 329-329
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- William A. Carlezon Jr., Rachael L. Neve
Pages 331-350
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Striatal Culture Preparation
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Front Matter
Pages 377-377
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- Steffany A. L. Bennett, Lysanne Melanson-Drapeau
Pages 397-404
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Detection of Transmitter Release in the Striatum
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Front Matter
Pages 413-413
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- James A. Zackheim, Elizabeth D. Abercrombie
Pages 433-441
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- Paul E. M. Phillips, Donita L. Robinson, Garret D. Stuber, Regina M. Carelli, R. Mark Wightman
Pages 443-464
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Behvioral Assessment and Others
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Front Matter
Pages 473-473
About this book
Drugs of Abuse: Neurological Reviews and Protocols is intended to provide insightful reviews of key current topics and, particularly, state-- the-art methods for examining drug actions in their various neuroanato- cal, neurochemical, neurophysiological, neuropharmacological, and molecular perspectives. The book should prove particularly useful to n- comers (graduate students and technicians) in this field, as well as to those established scientists (neuroscientists, biochemists, and molecular biologists) intending to pursue new careers or directions in the study of drugs. The book’s protocols cover a wide variety of coherent methods for gathering inf- mation on quantitative changes in proteins and mRNAs at both tissue and cel- lar levels. Inducible gene expression in striatal neurons has been a hot topic over the last decade. Alterations in gene expression for a wide range of proteins in the striatum have been investigated in response to drug administration. Altered expression of given mRNAs and their product proteins constitutes essential molecular steps in the development of neuroplasticity related to long-term addictive properties of drugs of abuse. With the multiple labeling methods that are also described in the book, gene expression can be detected in a chemically identified cell phenotype; the expression of multiple genes of interest can be detected in a single cell simultaneously. Hundreds or thousands of gene expr- sion products can today be detected in one experimental setup using the pow- ful systematic cDNA macroarray or microarray screening technology. Moreover, protocols useful in analyzing the functional roles of genes and proteins (e. g.
Reviews
"It describes in detail laboratory methods used by researchers working in the area of drug abuse and drug addiction. This is a unique book. Few books deal in detail with the methods underlying experiments. It will be of primary value to active investigators with active laboratories."-Doody's Health Sciences Book Review Journal
Editors and Affiliations
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School of Pharmacy, University of Missouri-Kansas City, Kansas, USA
John Q. Wang