Cancer Cell Signaling

Methods and Protocols

  • David M. Terrian

Part of the Methods in Molecular Biology™ book series (MIMB, volume 218)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Manipulation and Detection of Survival Signals

  3. Manipulation and Edtection of Oncogenic Signals

    1. Front Matter
      Pages 97-97
    2. Steven Pelech, Catherine Sutter, Hong Zhang
      Pages 99-111
    3. Julian Gomez-Cambronero, Joel Horwitz, Ramadan I. Sha’afi
      Pages 155-176
    4. Linda S. Steelman, William L. Blalock, Xiao-Yang Wang, Phillip W. Moye, John T. Lee, John G. Shelton et al.
      Pages 185-201
    5. Linda S. Steelman, William L. Blalock, Xiao-Yang Wang, Phillip W. Moye, John T. Lee, John G. Shelton et al.
      Pages 203-220
    6. Linda S. Steelman, William L. Blalock, Xiao-Yang Wang, Phillip W. Moye, John T. Lee, John G. Shelton et al.
      Pages 221-252

About this book

Introduction

The rapidly evolving nature of cell signaling research in cancer has necessitated a continuous updating of the methods used in its study, most recently to take into account the analysis of multiple members within a gene family. Cancer Cell Signaling: Methods and Protocols brings together all the major methods of cell research that are scientifically grounded within the cancer biology field. Presented in step-by-step detail to ensure successful results, the methods include proven techniques for the investigation of apoptosis and cell death, complementary protocols for manipulating and/or monitoring oncogenic signals in cancer cells, and techniques for studying protein-protein interactions. Finally, there is a detailed protocol for capturing pure samples of malignant cells from frozen tissue specimens and two alternative techniques for analyzing their genomic DNA. Each method is described by a hands-on master of the technique and includes invaluable notes on troubleshooting and pitfalls to avoid.
Cutting-edge and highly practical, Cancer Cell Signaling: Methods and Protocols provides today's cancer researchers with new, but well-proven, strategies and procedures for investigating how the signaling cascades essential to normal cell life are corrupted, as well as for developing new molecular tools for cancer therapy.

Editors and affiliations

  • David M. Terrian
    • 1
  1. 1.Brody School of MedicineEast Carolina UniversityGreenville

Bibliographic information

  • DOI https://doi.org/10.1385/1592593569
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-075-5
  • Online ISBN 978-1-59259-356-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book