Protein Sequencing Protocols

  • Bryan John Smith

Part of the Methods in Molecular Biology™ book series (MIMB, volume 211)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Bryan John Smith, Paul Tempst
    Pages 1-18
  3. Bryan John Smith
    Pages 49-61
  4. Bryan John Smith
    Pages 63-82
  5. G. Brent Irvine, Ian Davidson
    Pages 123-131
  6. Alan J. Smith
    Pages 133-141
  7. Andrea Scaloni, Maurizio Simmaco, Francesco Bossa
    Pages 169-180
  8. Andrew J. Reason
    Pages 181-194
  9. Fiona M. Greer, Howard R. Morris
    Pages 195-210

About this book


Bryan John Smith updates his much-acclaimed first edition of Protein Sequencing Protocols with new and refreshed techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to the analysis of protein sequence information via the powerful new tools of bioinformatics. Each readily reproducible protocol contains detailed step-by-step instructions to ensure successful experimental results, along with notes on troubleshooting and how to avoid pitfalls. Valuable appendices summarize changes in mass arising from protein modification, as well as consensus sequences for protein modification.
Comprehensive and up-to-date, Protein Sequencing Protocols, Second Edition, provides both novice and expert investigators alike a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination and thus help assure robust results.

Editors and affiliations

  • Bryan John Smith
    • 1
  1. 1.Celltech ChiroscienceSloughUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-975-9
  • Online ISBN 978-1-59259-342-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book