E. coliGene Expression Protocols

  • Peter E. Vaillancourt

Part of the Methods in Molecular Biology™ book series (MIMB, volume 205)

Table of contents

  1. Front Matter
    Pages i-xi
  2. François Baneyx, Mirna Mujacic
    Pages 1-18
  3. Rebecca L. Mullinax, David T. Wong, Heidi A. Davis, Kerstein A. Padgett, Joseph A. Sorge
    Pages 19-30
  4. Angelika Lueking, Sabine Horn, Hans Lehrach, Dolores J. Cahill
    Pages 31-42
  5. Samu Melkko, Dario Neri
    Pages 69-77
  6. Jeffrey D. Fox, David S. Waugh
    Pages 99-117
  7. Edward R. LaVallie, Elizabeth A. DiBlasio-Smith, Lisa A. Collins-Racie, Zhijian Lu, John M. McCoy
    Pages 119-140
  8. Rhesa D. Stidham, W. Christian Wigley, John F. Hunt, Philip J. Thomas
    Pages 155-170
  9. Thomas Lanio, Albert Jeltsch, Alfred Pingoud
    Pages 199-204
  10. Karen Johnston, Ronen Marmorstein
    Pages 205-213
  11. Karin A. Hughes, Jean P. Wiley
    Pages 215-223
  12. Leonardo Mariño-RamÍrez, Lisa Campbell, James C. Hu
    Pages 235-250
  13. Ernesto Abel-Santos, Charles P. Scott, Stephen J. Benkovic
    Pages 281-294

About this book

Introduction

Gene expression using E. coli as a host is carried out over a wide range of disciplines in academic and industrial laboratories. In E. coli Gene Expression Protocols, Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of
E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein, and those essential to functional genomics, proteomics, and protein engineering. Described in step-by-step detail to ensure robust, trouble-free results, each proven method has been written by a hands-on expert and includes extensive notes and practical tips for avoiding pitfalls. Even highly skilled researchers will find many time-saving techniques.
Authoritative and highly practical, E. coli Gene Expression Protocols provides a state-of-the-art collection of tested methods for this powerful gene expression technology, offering today's investigators proven tools for success in the emerging fields of functional genomics and proteomics.

Keywords

Promoter Proteomics biosynthesis gene expression genes tRNA transcription

Editors and affiliations

  • Peter E. Vaillancourt
    • 1
  1. 1.Applied Molecular EvolutionSan DiegoUSA

Bibliographic information

  • DOI https://doi.org/10.1385/1592593011
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-008-3
  • Online ISBN 978-1-59259-301-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book