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  • © 2002

Peptide Research Protocols

Endothelin

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Part of the book series: Methods in Molecular Biology (MIMB, volume 206)

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  • ISBN: 978-1-59259-289-0
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Table of contents (14 protocols)

  1. Front Matter

    Pages i-x
  2. Endothelin Receptor Protocols

    1. Front Matter

      Pages 43-43
    2. Quantification of Endothelin Receptor mRNA by Competitive RT-PCR

      • Paula J. W. Smith, Juan Carlos Monge
      Pages 93-104
  3. Endothelin-Converting Enzyme Protocols

    1. Front Matter

      Pages 105-105
    2. Detection and Assessment of Endothelin-Converting Enzyme Activity

      • Carolyn D. Jackson, Anthony J. Turner
      Pages 107-124
    3. Quantitative Measurement of mRNA Levels by RT-PCR

      • Delphine M. Lees, Noorafza Q. Khan, Stewart Barker, Roger Corder
      Pages 125-145
  4. In Vitro Functional Assays

    1. Front Matter

      Pages 179-179
  5. In Vivo Functional Assays

    1. Front Matter

      Pages 215-215
    2. Investigation of the Endothelin System in Experimental Heart Failure

      • Gillian A. Gray, Lorcan Sherry
      Pages 217-227

About this book

Tantalizing new research frontiers are opening in peptide research, where the endothelins constitute an important class of signals that can be manipulated therapeutically to treat cardiovascular disease. In Peptide Research Protocols: Endothelin, a panel of multidisciplinary experts describes in detail readily reproducible methods to investigate all aspects of peptide research by focusing on the endothelin system-from its synthesis and metabolism, to its function in health and disease. These methods use state-of-the-art molecular techniques to quantify the expression of mRNA, here using both endothelin receptors and the endothelin-converting enzymes. They show how peptides, precursors, receptors, and synthetic enzymes can be localized and quantified in plasma, culture supernatants, tissue homogenate, and tissue sections using antibodies, and how radioligand binding techniques can provide additional information on receptor characterization. In vitro assays are presented to determine the function of peptides in isolated preparations, to characterize new receptor ligands, and to provide information on the tissue-specific processing of precursor peptides-all in the endothelin system. Several in vivo protocols illustrate the role of the endothelin peptides in healthy human individuals and describe animal models that can be used to predict the therapeutic potential of cardiovascular drugs that manipulate endothelin synthesis or function.
Authoritative and timely, Peptide Research Protocols: Endothelin provides both a comprehensive introduction to endothelin biology and an up-to-date collection of proven techniques and experimental strategies for use in peptide research today.

Reviews

"This book is excellent for what it intends to be: to provide a coherent series of protocols on a focused topic. It efficiently integrates biochemical, pharmacological, physiological and molecular biological methods..." -Acta Cardiologica

Editors and Affiliations

  • Clinical Pharmacology Unit, University of Cambridge, Cambridge, UK

    Janet J. Maguire, Anthony P. Davenport

Bibliographic Information

  • Book Title: Peptide Research Protocols

  • Book Subtitle: Endothelin

  • Editors: Janet J. Maguire, Anthony P. Davenport

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592899

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2002

  • Hardcover ISBN: 978-0-89603-993-3

  • Softcover ISBN: 978-1-61737-291-9

  • eBook ISBN: 978-1-59259-289-0

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 242

  • Topics: Pharmacy

Buying options

eBook
USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-59259-289-0
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 159.00
Price excludes VAT (USA)
Hardcover Book
USD 159.99
Price excludes VAT (USA)