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Antibody Phage Display

Methods and Protocols

  • Philippa M. O’Brien
  • Robert Aitken

Part of the Methods in Molecular Biology™ book series (MIMB, volume 178)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Philippa M. O’Brien, Robert Aitken
    Pages 73-86
  3. Hans J.W de Haard
    Pages 87-100
  4. Jacqueline Sharon, Seshi R. Sompuram, Chiou-Ying Yang, Brent R. Williams, Stefanie Sarantopoulos
    Pages 101-112
  5. David W. J. Coomber
    Pages 133-145
  6. Patrick Chames, Hennie R. Hoogenboom, Paula Henderikx
    Pages 147-157
  7. Kunihiko Itoh, Toshio Suzuki
    Pages 195-199
  8. Jane K. Osbourn
    Pages 201-205
  9. Elvyra J. Noronha, Xinhui Wang, Soldano Ferrone
    Pages 227-233
  10. Katarina Radosevic, Willem van Ewijk
    Pages 235-243
  11. Ann-Christin Malmborg, Nina Nilsson, Mats Ohlin
    Pages 245-253
  12. Yoshitaka Iba, Chie Miyazaki, Yoshikazu Kurosawa
    Pages 259-267
  13. Robertn A. Irving, Gregory Coia, Anna Raicevic, Peter J. Hudson
    Pages 295-302
  14. Johan Lantto, Pernilla Jirholt, Yvelise Barrios, Mats Ohlin
    Pages 303-316
  15. Sergey M. Kipriyanov
    Pages 317-331
  16. Pascale A. Cohen
    Pages 367-378
  17. Michelle de Graaf, Ida H. van der Meulen-Muileman, Herbert M. Pinedo, Hidde J. Haisma
    Pages 379-387
  18. Back Matter
    Pages 397-401

About this book

Introduction

Antibody phage display, the definitive technology for monoclonal production, has now advanced to a stage where it can be performed in nonspecialized research laboratories. In Antibody Phage Display: Methods and Protocols, Philippa M. O'Brien and Robert Aitken combine in one volume a comprehensive collection of established antibody phage display protocols, each accompanied by authoritative guidance that will enable the nonspecialist to carry them out successfully. Coverage spans the construction of antibody libraries, the selection of antibody clones with the desired properties, and their modification, expression, and purification. Each readily reproducible method is described by a hands-on expert in step-by-step detail and includes a wealth of practical advice not found in the scientific literature. Extensive notes discuss pitfalls to avoid and offer many possible alternative methods to suit special research situations. An overview by one of the world's leading experts in antibody phage display, Dr. Hennie R. Hoogenboom, surveys the current status of the field and the future of the technology.
Comprehensive and highly practical, Antibody Phage Display: Methods and Protocols provides biochemists, molecular biologists, and immunologists with a gold-standard reference guide to the successful isolation, modification, and expression of recombinant antibodies using today's powerful phage display technology.

Editors and affiliations

  • Philippa M. O’Brien
    • 1
  • Robert Aitken
    • 1
  1. 1.University of GlasgowGlasgowScotland, UK

Bibliographic information

  • DOI https://doi.org/10.1385/1592592406
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-906-3
  • Online ISBN 978-1-59259-240-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site