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  • © 2001

Genomics Protocols

Part of the book series: Methods in Molecular Biology (MIMB, volume 175)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xiv
  2. Genetic Analysis of Complex Traits

    • Stephen P. Bryant, Mathias N. Chiano
    Pages 11-27
  3. Sequence-Based Detection of Single Nucleotide Polymorphisms

    • Deborah A. Nickerson, Natali Kolker, Scott L. Taylor, Mark J. Rieder
    Pages 29-35
  4. Genomic Mismatch Scanning for the Mapping of Genetic Traits

    • Farideh Mirzayans, Michael A. Walter
    Pages 37-46
  5. Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization

    • Mario A. J. A. Hermsen, Marjan M. Weiss, Gerrit A. Meijer, Jan P. A. Baak
    Pages 47-55
  6. Construction of a Bacterial Artificial Chromosome Library

    • Sangdun Choi, Ung-Jin Kim
    Pages 57-68
  7. Contiguation of Bacterial Clones

    • Sean J. Humphray, Susan J. Knaggs, Ioannis Ragoussis
    Pages 69-108
  8. Map Integration

    • Panagiotis Deloukas
    Pages 129-142
  9. Construction of Full-Length-Enriched cDNA Libraries

    • Yutaka Suzuki, Sumio Sugano
    Pages 143-153
  10. Preparation and Screening of High-Density cDNA Arrays with Genomic Clones

    • Günther Zehetner, Maria Pack, Katja Schäfer
    Pages 169-188
  11. Direct Selection of cDNAs by Genomic Clones

    • Daniela Toniolo
    Pages 189-199
  12. Exon Trapping

    • Martin C. Wapenaar, Johan T. Den Dunnen
    Pages 201-215
  13. Sequencing Bacterial Artificial Chromosomes

    • David E. Harris, Lee Murphy
    Pages 217-234
  14. Finding Genes in Genomic Nucleotide Sequences by Using Bioinformatics

    • Yvonne J. K. Edwards, Simon M. Brocklehurst
    Pages 235-247

About this book

We must unashamedly admit that a large part of the motivation for editing Genomics Protocols was selfish. The possibility of assembling in a single volume a unique and comprehensive collection of complete protocols, relevant to our work and the work of our colleagues, was too good an opportunity to miss. We are pleased to report, however, that the outcome is something of use not only to those who are experienced practitioners in the genomics field, but is also valuable to the larger community of researchers who have recognized the potential of genomics research and may themselves be beginning to explore the technologies involved. Some of the techniques described in Genomics Protocols are clearly not restricted to the genomics field; indeed, a prerequisite for many procedures in this discipline is that they require an extremely high throughput, beyond the scope of the average investigator. However, what we have endeavored here to achieve is both to compile a collection of procedures concerned with geno- scale investigations and to incorporate the key components of “bottom-up” and “top-down” approaches to gene finding. The technologies described extend from those traditionally recognized as coming under the genomics umbrella, touch on proteomics (the study of the expressed protein complement of the genome), through to early therapeutic approaches utilizing the potential of genome programs via gene therapy (Chapters 27–30).


"The two editors of this volume have invited contributors from a variety of authors to write a laboratory manual dealing exclusively with the application of genomic methods in eukaryotes....Each chapter is presented in a standardized fashion, beginning with an introduction followed by a list of all the materials needed (including the machines and software) a detailed experimental protocol, notes (troubleshooting), and a list of will be of value for those requiring step-by-step "cookbook" procedures for some of the methodologies described, and this definitive and authorative volume belongs in the library of every quality research institution where genomics is performed." - CHEMBIOCHEM

"...a solid introduction to the basic techniques involved in the process of disease gene identification...should prove to be useful to those scientists who wish to expand their skills in the field." -Biotech Software & Internet Report

Editors and Affiliations

  • UK Human Genome Mapping Project Resource Centre, Cambridge, UK

    Michael P. Starkey, Ramnath Elaswarapu

Bibliographic Information

  • Book Title: Genomics Protocols

  • Editors: Michael P. Starkey, Ramnath Elaswarapu

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2001

  • eBook ISBN: 978-1-59259-235-7Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 538

  • Number of Illustrations: 113 b/w illustrations, 5 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access