Skip to main content
SpringerLink
Log in
Book cover
  • Book
  • © 2000

Septic Shock Methods and Protocols

Editors:

  1. Thomas J. Evans

    1. Imperial College School of Medicine, London, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 36)

Sections

Buy it now

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
    PDF

  2. Endotoxin

    1. Front Matter

      Pages 1-1
      PDF

    2. Assay of Endotoxin by Limulus Amebocyte Lysate

      • Paul A. Ketchum, Thomas J. Novitsky
      Pages 3-12

    3. Preparation of Endotoxin from Pathogenic Gram-Negative Bacteria

      • Alexander Shnyra, Michael Luchi, David C. Morrison
      Pages 13-25

    4. Assay of Anti-Endotoxin Antibodies

      • Lore Brade
      Pages 27-35

  3. Other Bacterial Products

    1. Front Matter

      Pages 57-57
      PDF

    2. Purification of Streptococcal Pyrogenic Exotoxin A

      • Manuela Roggiani, Patrick M. Schlievert
      Pages 59-66

    3. Assay for Superantigens

      • Shiranee Sriskandan
      Pages 67-79

  4. Cytokines

    1. Front Matter

      Pages 81-81
      PDF

    3. Assay of Soluble Tumor Necrosis Factor Receptors

      • Maarten G. Bouma, Wim A. Buurman
      Pages 91-100

    4. Whole-Blood Assays for Cytokine Production

      • Daniel G. Remick, David E. Newcomb, Jon S. Friedland
      Pages 101-112

  5. Nitric Oxide and Other Reactive Nitrogen Intermediates

    1. Front Matter

      Pages 113-113
      PDF

    2. Nitric Oxide Synthase Inhibitors

      • Dilani K. Siriwardena, Hajime Tagori, Christoph Thiemermann
      Pages 115-131

    3. In Situ Detection of Nitric Oxide

      • Tadeusz Malinski
      Pages 133-144

    4. Immunochemical Detection of Nitric Oxide Synthase in Human Tissue

      • Lee D. K. Buttery, Julia M. Polak
      Pages 145-157

    5. Anti-Nitrotyrosine Antibodies for Immunohistochemistry

      • Liliana Viera, Yao Zu Ye, Joseph S. Beckman
      Pages 159-169

    6. Detection of Peroxynitrite in Biological Fluids

      • Stuart Malcolm, Raymond Foust III, Caryn Hertkorn, Harry Ischiropoulos
      Pages 171-177
Back to top

About this book

Septic shock remains a serious medical condition with high mortality. Despite many advances in intensive care medicine and antibiotic devel- ment, this has not changed appreciably in the last 20 years. Frustratingly, over the same period of time, enormous advances have been made in understa- ing the underlying pathogenic mechanisms of this condition. This has resulted in the development of several novel therapies for septic shock, which, despite excellent theoretical grounds for their efficacy, have failed in altering mort- ity attributable to sepsis. The reasons for these failures are multiple, but it is clear that further research is required aimed at increasing our understanding of the basic pat- physiological processes that occur following infection. Research into septic shock draws upon a number of different disciplines, ranging from molecular and cellular biology to physiological measurements on whole animals. Septic Shock Methods and Protocols is an attempt to draw together into one volume a number of protocols that are of use in the investigation of the mechanisms of septic shock. I have divided the book into five sections. The first deals with endotoxin, the lipopolysaccharide component of the Gram-negative cell m- brane that can mimic many of the features of septic shock. Gram-positive organisms are found increasingly as causes of septic shock, and several - pects of toxins produced from these bacteria are considered in the second s- tion.
Back to top

Keywords

Back to top

Reviews

"this text is a fine laboratory reference. . . the editor has done an excellent job in the organization of the text and has selected well-qualified investigators who have made a commendable contribution to the various sections of the book. This text is appropriate for a broad-range of interdisciplinary investigators and students engaged in septic shock/infection studies. "-Doody's Health Sciences Book Review Journal
Back to top

Editors and Affiliations

  • Imperial College School of Medicine, London, UK

    Thomas J. Evans

Back to top

Bibliographic Information

  • Book Title: Septic Shock Methods and Protocols

  • Editors: Thomas J. Evans

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592592163

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2000

  • Hardcover ISBN: 978-0-89603-730-4Published: 03 December 1999

  • Softcover ISBN: 978-1-61737-154-7Published: 09 November 2010

  • eBook ISBN: 978-1-59259-216-6Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: X, 208

  • Topics: Infectious Diseases

Back to top

Publish with us

Policies and ethics

Back to top
Access via your institution

Buy it now

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Search

Navigation