Advertisement

Genomic Imprinting

Methods and Protocols

  • Andrew Ward

Part of the Methods in Molecular Biology™ book series (MIMB, volume 181)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Wendy L. Dean, Gavin Kelsey, Wolf Reik
    Pages 1-19
  3. Anne C. Ferguson-Smith, Maxine Tevendale, Pantelis Georgiades, Valerie Grandjean
    Pages 41-54
  4. Justin F.-X. Ainscough, Rosalind M. John, Sheila C. Barton
    Pages 55-65
  5. Rosalind M. John, Justin F.-X. Ainscough, Sheila C. Barton
    Pages 67-81
  6. Izuho Hatada, Tsunehiro Mukai
    Pages 83-100
  7. Fumitoshi Ishino, Yoshimi Kuroiwa, Naoki Miyoshi, Shin Kobayashi, Takashi Kohda, Tomoko Kaneko-Ishino
    Pages 101-112
  8. Rachel J. Smith, Gavin Kelsey
    Pages 113-132
  9. Joanne L. Thorvaldsen, Marisa S. Bartolomei
    Pages 133-144
  10. Judith Singer-Sam, Chunguang Gao
    Pages 145-152
  11. Rolf Ohlsson, Kristian Svensson, Hengmi Cui, Helena Malmikumpu, Gail Adam
    Pages 153-167
  12. Giovanna Braidotti
    Pages 169-180
  13. Sabine Engemann, Osman El-Maarri, Petra Hajkova, Joachim Oswald, Joern Walter
    Pages 217-228
  14. Déborah Bourc’his, Evani Viegas-Péquignot
    Pages 229-242
  15. Ilse Van den Wyngaert, Roger L. P. Adams, Stefan U. Kass
    Pages 243-250
  16. Ghislaine Dell, Marika Charalambous, Andrew Ward
    Pages 251-258
  17. Richard I. Gregory, Sanjeev Khosla, Robert Feil
    Pages 269-284
  18. Ji-Fan Hu, Andrew R. Hoffman
    Pages 285-296
  19. Peter L. Jones, Paul A. Wade, Alan P. Wolffe
    Pages 297-307
  20. Kiyoe Ura, Yasufumi Kaneda
    Pages 309-325
  21. Rinke Vinkenoog, Melissa Spielman, Sally Adams, Hugh G. Dickinson, Rod J. Scott
    Pages 327-370
  22. Back Matter
    Pages 371-377

About this book

Introduction

Imprinted genes, many of which generally control growth and development, frequently lose their imprints during cancer progression, a loss that then plays a substantial role in uncontrolled tumor growth. Imprint instability also appears to be a major limitation to the success of mammalian cloning experiments. In Genomic Imprinting: Methods and Protocols, Andrew Ward and a team of experienced researchers have brought together a collection of optimized classic and vanguard techniques for the identification and analysis of imprinted genes. The majority of protocols describe molecular techniques that allow examination of gene structure or expression in an allele-specific manner. Protocols are included for identifying and cloning imprinted genes, for analyzing imprinted gene expression, for the study of DNA methylation and methylation-sensitive DNA-binding proteins, and for examining chromatin structure. There are also methods for the manipulation of mouse embryos to produce monoparental embryos and embryonic stem cells, and for the generation of transgenic mice with BAC, PAC, and YAC constructs. Each technique is described in step-by-step detail to ensure successful results.
Incorporating a wealth of knowledge from leading exponents in the field, Genomic Imprinting: Methods and Protocols brings together all the essential molecular, genetic, and embryological methods commonly used in today's laboratories for the identification and analysis of imprinted genes.

Editors and affiliations

  • Andrew Ward
    • 1
  1. 1.Department of Biology and BiochemistryUniversity of BathClaverton DownUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592592112
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-741-0
  • Online ISBN 978-1-59259-211-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site