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Proteoglycan Protocols

  • Renato V. Iozzo

Part of the Methods in Molecular Biology™ book series (MIMB, volume 171)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Isolation and Purification

    1. Front Matter
      Pages 1-1
    2. Kathryn G. Vogel, Julie A. Peters
      Pages 9-17
    3. William D. Staatz, Hidenao Toyoda, Akiko Kinoshita-Toyoda, Kimberlly Chhor, Scott B. Selleck
      Pages 41-52
    4. Geetha Sugumaran, Jeremiah E. Silbert
      Pages 103-116
    5. Anna H. K. Plaas, L. West, Ronald J. Midura, Vincent C. Hascall
      Pages 117-128
    6. Madeleine Karlsson, Sven Björnsson
      Pages 159-173
    7. Yanusz Wegrowski, Francois-Xavier Maquart
      Pages 175-179
  3. Expression, Detection, and Degradation

    1. Front Matter
      Pages 199-199
    2. David J. McQuillan, Neung-Seon Seo, Anne M. Hocking, Camille I. McQuillan
      Pages 201-219
    3. Anna L. McBain, David M. Mann
      Pages 221-229
    4. Alan D. Murdoch, Renato V. Iozzo
      Pages 231-238
    5. Giancarlo Ghiselli, Renato V. Iozzo
      Pages 239-250
    6. Melissa Handler, Renato V. Iozzo
      Pages 251-259
    7. Jens W. Fischer, Michael G. Kinsella, David Hasenstab, Alexander W. Clowes, Thomas N. Wight
      Pages 261-269
    8. Stephanie L. Lara, Stephen P. Evanko, Thomas N. Wight
      Pages 271-290
    9. Wei Luo, Chunxia Guo, Jing Zheng, Marvin L. Tanzer
      Pages 291-300
    10. Tung-Ling L. Chen, Peiyin Wang, Seung S. Gwon, Nina W. Flay, Barbara M. Vertel
      Pages 301-308
    11. Xiaomei Bai, Brett Crawford, Jeffrey D. Esko
      Pages 309-316
    12. John R. Couchman, Pairath Tapanadechopone
      Pages 329-333
    13. H. Edward Conrad
      Pages 347-351
    14. Laurie A. LeBrun, Robert J. Linhardt
      Pages 353-361
    15. MarÍa José Hernáiz, Robert J. Linhardt
      Pages 363-371
    16. Andrew P. Spicer
      Pages 373-382
    17. Susan Stair Nawy, Antonei B. Csóka, Kazuhiro Mio, Robert Stern
      Pages 383-389
    18. Kazuhiro Mio, Antonei B. Csóka, Susan Nawy Stair, Robert Stern
      Pages 391-397
  4. Interacations

    1. Front Matter
      Pages 399-399
    2. James D. San Antonio, Arthur D. Lander
      Pages 401-414
    3. David K. Moscatello, Renato V. Iozzo
      Pages 427-434
    4. John B. Ancsin, Robert Kisilevsky
      Pages 449-456
    5. Kevin Jon Williams
      Pages 457-477
    6. Bryan P. Toole, Qin Yu, Charles B. Underhill
      Pages 479-485

About this book

Introduction

Proteoglycans, highly complex macromolecules found in mammalian and lower organisms, serve a multitude of biological functions. In Proteoglycan Protocols, Renato Iozzo, MD, and an authoritative team of investigators present for the first time a comprehensive and up-to-date collection of readily reproducible preparative and analytical methods for the in-depth analysis of these important compounds. Featuring step-by-step protocols, this book will enable both novice and experienced researchers to isolate intact proteoglycans from tissues and cultured cells, to establish the composition of their carbohydrate moieties, and to generate strategies for prokaryotic and eukaryotic expression. There are also detailed techniques for the suppression of specific proteoglycan genes, for the detection of mutant cells and their degradation products, and for studying specific interactions between proteoglycans and extracellular matrix proteins and with growth factors and their receptors. Each method is carefully described by leading experts in the field and includes practical considerations that are often lacking in formal papers.

Authoritative and detailed, Proteoglycan Protocols offers molecular and cellular biologists, biochemists, pathologists, and geneticists a comprehensive reference and practical guide to the most common methods used today for the isolation and characterization of proteoglycans from cultured cells, tissues, and biological fluids.

Editors and affiliations

  • Renato V. Iozzo
    • 1
  1. 1.Department of Pathology, Anatomy, and Cell BiologyThomas Jefferson UniversityPhiladelphia

Bibliographic information

  • DOI https://doi.org/10.1385/1592592090
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-759-5
  • Online ISBN 978-1-59259-209-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site