DNA'Protein Interactions

1. Front Matter

   Pages i-xvii

   PDF

2. Filter-Binding Assays

   • Peter G. Stockley

   Pages 1-11

3. Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions

   • Marc-André Laniel, Alain Béliveau, Sylvain L. Guérin

   Pages 13-30

4. DNase I Footprinting

   • Benoît Leblanc, Tom Moss

   Pages 31-38

5. Footprinting with Exonuclease III

   • Willi Metzger, Hermann Heumann

   Pages 39-47

6. Hydroxyl Radical Footprinting

   • Evgeny Zaychikov, Peter Schickor, Ludmilla Denissova, Hermann Heumann

   Pages 49-61

7. The Use of Diethyl Pyrocarbonate and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA

   • Brenda F. Kahl, Marvin R. Paule

   Pages 63-75

8. Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper

   • Athanasios G. Papavassiliou

   Pages 77-110

9. Uranyl Photofootprinting

   • Peter E. Nielsen

   Pages 111-119

10. Osmium Tetroxide Modification and the Study of DNA-Protein Interactions

    • James A. McClellan

    Pages 121-134

11. Determination of a Transcription-Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots

    • Athanasios G. Papavassiliou

    Pages 135-149

12. Diffusible Singlet Oxygen as a Probe of DNA Deformation

    • Malcolm Buckle, Andrew A. Travers

    Pages 151-160

13. Ultraviolet-Laser Footprinting

    • Johannes Geiselmann, Frederic Boccard

    Pages 161-173

14. In Vivo DNA Analysis

    • Régen Drouin, Jean-Philippe Therrien, Martin Angers, Stéphane Ouellet

    Pages 175-219

15. Identification of Protein-DNA Contacts with Dimethyl Sulfate

    • Peter E. Shaw, A. Francis Stewart

    Pages 221-227

16. Ethylation Interference

    • Ian W. Manfield, Peter G. Stockley

    Pages 229-243

17. Hydroxyl Radical Interference

    • Peter Schickor, Evgeny Zaychikov, Hermann Heumann

    Pages 245-253

18. Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting

    • Simon Labbé, Gale Stewart, Olivier LaRochelle, Carl Séguin, Guy G. Poirier

    Pages 255-264

19. A Competition Assay for DNA Binding Using the Fluorescent Probe ANS

    • Ian A. Taylor, G. Geoff Kneale

    Pages 265-274

20. Site-Directed Cleavage of DNA by Linker Histone-Fe(II) EDTA Conjugates

    • David R. Chafin, Jeffrey J. Hayes

    Pages 275-290

Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.

• DNA
• RNA
• Termination
• Vivo
• Western Blotting
• bacteria
• cell
• development
• gene
• genes
• nucleic acid
• protein
• reproducible techniques
• transcription

"The book, written by an impressive list of worldwide contributors, will provide readers with nearly all the necessary tools for studying DNA-protein interactions, making it a perfect cell laboratory manual." -Quarterly Review of Biology

"...a great laboratory resource for biochemists and molecular, cellular, and developmental biologists." - Today's Life Science

• Centre de Recherche en Cancérologie de l’Université Laval, Centre Hopital Universitaire de Québec et Départment de biologie médicale, Université Laval, Québec, Canada

  Tom Moss

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