In Vitro Mutagenesis Protocols

  • Jeff Braman

Part of the Methods in Molecular Biology™ book series (MIMB, volume 182)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Priya Handa, Swapna Thanedar, Umesh Varshney
    Pages 1-6
  3. Christine A. Andrews, Scott A. Lesley
    Pages 7-17
  4. Yang-Gyun Kim, Stefan Maas
    Pages 29-36
  5. Xueni Chen, Weimin Liu, Ileana Quinto, Giuseppe Scala
    Pages 67-70
  6. Jesper Brøs-Poulsen, Jane Nøhr, Leif Kongskov Larsen
    Pages 71-76
  7. Albert Jeltsch, Thomas Lanio
    Pages 85-94
  8. Amom Ruhikanta Meetei, M. R. S. Rao
    Pages 95-102
  9. Ross N. Nazar, P. D. Abeyrathne, Robert V. A. Intine
    Pages 117-126
  10. Dario E. Lehoux, Roger C. Levesque
    Pages 127-137
  11. Jennifer A. Maynard, Gang Chen, George Georgiou, Brent L. Iverson
    Pages 149-163
  12. French A. Lewis, Brian A. Dougherty
    Pages 173-187

About this book

Introduction

In the post-genome era, in vitro mutagenesis has emerged as the critically important tool used by molecular biologists in establishing the functions of components of the proteome. In this second edition of In Vitro Mutagenesis Protocols, active researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. Each contributor focuses on improvements to conventional site-directed mutagenesis, with chapters being devoted to chemical site-directed mutagenesis; PCR-based mutagenesis and the modifications that allow high-throughput experiments; and mutagenesis based on gene disruption that is both in vitro- and in situ-based. Additional methods are provided for in vitro gene evolution; for gene disruption based on transposon, recombination, and cassette mutagenesis; and for facilitating the introduction of multiple mutations. Each readily reproducible technique includes detailed step-by-step instructions, tips on pitfalls to avoid, and notes on reagents and suppliers.


Time-tested and highly practical, the techniques in In Vitro Mutagenesis Protocols, Second Edition offer today's molecular biologists a rich compendium of reliable and powerful techniques with which to illuminate the proteome.

Editors and affiliations

  • Jeff Braman
    • 1
  1. 1.StratageneLa Jolla

Bibliographic information

  • DOI https://doi.org/10.1385/1592591949
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-910-0
  • Online ISBN 978-1-59259-194-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book