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Epithelial Cell Culture Protocols

  • Clare Wise

Part of the Methods In Molecular Medicine™ book series (MIMB, volume 188)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Nobuhiro Ibaraki
    Pages 1-6
  3. Mutsuo Yamaya, Masayoshi Hosoda, Tomoko Suzuki, Norihiro Yamada, Hidetada Sasaki
    Pages 7-16
  4. Yoshitaka Konda, Tsutomu Chiba
    Pages 17-25
  5. Carsten Röpke
    Pages 27-36
  6. Alphonse E. Sirica
    Pages 37-52
  7. Rolf Gebhardt
    Pages 53-63
  8. Ben Forbes
    Pages 65-75
  9. Wei Zheng, Qiuqu Zhao
    Pages 99-114
  10. Philip H. Karp, Thomas O. Moninger, S. Pary Weber, Tamara S. Nesselhauf, Janice L. Launspach, Joseph Zabner et al.
    Pages 115-137
  11. Kairbaan Hodivala-Dilke
    Pages 139-144
  12. Andrew P. Gilmore, Charles H. Streuli
    Pages 145-155
  13. David L. Hudson
    Pages 157-167
  14. Joachim Rychlyv
    Pages 169-177
  15. Minoru Ueda, Yukio Sumi, Yoshitaka Hibino, Ken-Ichiro Hata
    Pages 197-211
  16. Staffan Tavelin, Johan Gråsjö, Jan Taipalensuu, Göran Ocklind, Per Artursson
    Pages 233-272
  17. Godfried M. Roomans
    Pages 273-289
  18. George Stoica
    Pages 305-314
  19. Carol A. Bertrand, Ulrich Hopfer
    Pages 315-327
  20. Derek M. McKay, Mary H. Perdue
    Pages 359-371
  21. Carin Sahlberg, Tuija Mustonen, Irma Thesleff
    Pages 373-382
  22. Nicola Jones, Mary H. Perdue, Philip M. Sherman, Derek M. McKay
    Pages 383-400
  23. Back Matter
    Pages 401-405

About this book

Introduction

Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. In Epithelial Cell Culture Protocols, a team of well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. These readily reproducible techniques describe all the commonly used protocols, as well as those that are more specialized, including several aimed at clinical investigation. The emphasis is on the analysis and assessment of epithelial cells, for example, by looking at apoptosis and integrins or by measuring membrane capacitance and confluence. Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. Several protocols cover the culturing of epithelial cells and their use in treating patients with burns and other skin disorders.
Wide-ranging and highly practical, Epithelial Cell Culture Protocols offers both novices and expert investigators alike a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms in general, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells.

Editors and affiliations

  • Clare Wise
    • 1
  1. 1.Medical Research CouncilNational Institute for Medical ResearchLondonUK

Bibliographic information

  • DOI https://doi.org/10.1385/159259185X
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-893-6
  • Online ISBN 978-1-59259-185-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site