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Transgenesis Techniques

Principles and Protocols

  • Alan R. Clarke

Part of the Methods in Molecular Biology book series (MIMB, volume 180)

Table of contents

  1. Front Matter
    Pages i-x
  2. Topical Reviews in Transgenesis

    1. Front Matter
      Pages 1-23
  3. Transgenesis in Invertebrate and Lower Vertebrate Species

    1. Front Matter
      Pages 25-36
    2. Mark J. O’Connor, William Chia
      Pages 27-36
  4. Transgenesis in the Mouse: Oocyte Injection

    1. Front Matter
      Pages 37-70
    2. Gary A. J. Brown, Timothy J. Corbin
      Pages 39-70
  5. Alternative Routes to the Germline

    1. Front Matter
      Pages 71-89
    2. Tohru Tsukui, Yutaka Toyoda
      Pages 73-82
    3. Richard A. Bowen
      Pages 83-90
  6. Transgenesis in the Mouse: The ES Cell Route

    1. Front Matter
      Pages 91-204
    2. David W. Melton
      Pages 151-173
    3. Ralf Kühn, Raul M. Torres
      Pages 175-204
  7. Cryopreservation of Mouse Lines

    1. Front Matter
      Pages 205-251
    2. Jillian M. Shaw, Naomi Nakagata
      Pages 207-228
    3. Jillian M. Shaw, Alan O. Trounson
      Pages 229-252
  8. Transgenesis in the Rat

    1. Front Matter
      Pages 253-341
    2. Linda J. Mullins, Gillian Brooker, John J. Mullins
      Pages 255-270
  9. Transgenesis in Domestic Species

    1. Front Matter
      Pages 271-271
    2. Keith H. S. Campbell
      Pages 289-301
  10. Characterization and Analysis of Transgenic Strains

    1. Front Matter
      Pages 303-303
    2. Stefan Selbert, Dominic Rannie
      Pages 305-341
  11. Back Matter
    Pages 343-351

About this book

Introduction

The past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to present a collection of readily reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes much space to the creation of genetically modified murine strains, providing access to the germline both by conventional pronuclear injection and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms.
Comprehensive and highly practical, this second edition of Transgenesis Techniques: Principles and Protocols updates and expands the acclaimed first edition with a wealth of easy-to-follow cutting-edge protocols and practical tips for producing transgenic animals and analyzing their integrated transgenes.

Editors and affiliations

  • Alan R. Clarke
    • 1
  1. 1.School of Biosciences, Cardiff UniversityCardiffUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592591787
  • Copyright Information Humana Press 2002
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-696-3
  • Online ISBN 978-1-59259-178-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site