Oxidative Stress Biomarkers and Antioxidant Protocols

  • Donald Armstrong

Part of the Methods in Molecular Biology™ book series (MIMB, volume 186)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Techniques for Free Radical Derived Biomarkers

    1. Front Matter
      Pages 1-1
    2. Maria Giulia Battelli, Silvia Musiani
      Pages 3-11
    3. Douglas Borchman, Santosh Sinha
      Pages 21-28
    4. Hiroshi Ohshima, László. Virág, Jose Souza, Vladimir Yermilov, Brigitte Pignatelli, Mitsuharu Masuda et al.
      Pages 77-88
    5. José A. Castro, Gerardo D. Castro
      Pages 89-99
    6. Bénédicte Morin, Shanlin Fu, Hongjie Wang, Michael J. Davies, Roger T. Dean
      Pages 101-110
    7. Aqeela Afzal, Mohammed Afzal, Andrew Jones, Donald Armstrong
      Pages 111-115
    8. Mohammed Afzal, Aqeela Afzal, Andrew Jones, Donald Armstrong
      Pages 117-122
    9. I. Hendrikje Buss, Christine C. Winterbourn
      Pages 123-128
    10. J. Nikki Shaw, John W. Baynes, Suzanne R. Thorpe
      Pages 129-137
    11. Andrew R. Collins, Mária Dušinská
      Pages 147-159

About this book

Introduction

Oxidative stress has been linked to a variety of medical problems, including cardiovascular disease, cancer, arthritis, diabetes, and aging. Drawing on the growing volume of research into the impact of oxidative stress on pathophysiological mechanisms, Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the perturbations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format by accomplished investigators from prestigious universities, institutes, and laboratories around the world, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltammetry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols (Humana, 2002), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays.
Cutting-edge and highly practical, Oxidative Stress Biomarkers and Antioxidant Protocols provides many powerful new tools for detecting oxidative stress and for investigating the possibilities for therapeutic intervention monitoring in a variety of serious diseases.

Editors and affiliations

  • Donald Armstrong
    • 1
  1. 1.University of FloridaGainesville

Bibliographic information

  • DOI https://doi.org/10.1385/1592591736
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-850-9
  • Online ISBN 978-1-59259-173-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book